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The mitochondrial genomes of Crispatotrochus rubescens and Crispatotrochus rugosus (Hexacorallia; Scleractinia): new insights on the phylogeny of the family Caryophylliidae

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Abstract

Background

Caryophylliidae is one of the most diverse scleractinian families, however it was recovered as polyphyletic in multiple molecular studies. Recently, the mitochondrial gene order was proposed as a character for a taxonomic revision of the family. Here we describe the first mitogenome of the caryophylliid genus Crispatotrochus, whose phylogenetic position remains uncertain.

Methods and results

The complete mitochondrial genomes of Crispatotrochus rubescens and Crispatotrochus rugosus were sequenced, assembled, and annotated. The two mitogenomes are identical and circular, have a length of 16,536 bp, a GC content of 35.9%, and contain 13 protein-coding genes, 2 ribosomal RNAs and 2 transfer RNAs. Both species have a transposition of a three gene block - cob, nad2, and nad6 - similarly to a group of caryophylliid genera that were recovered as monophyletic, including the type genus (Caryophyllia) of the family. The phylogenetic analyses recovered Crispatotrochus within the clade that presents the gene rearrangement and specifically as sister taxa of the genus Caryophyllia, a result consistent with previous studies and the similar gross morphology of the two genera.

Conclusions

We determined the mitochondrial genomes of the genus Crispatotrochus to investigate their relations within Scleractinia. Results from this study provide insights on the phylogenetic position of the genus and corroborate that the mitochondrial gene order could be used as taxonomic character for the family Caryophylliidae.

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Acknowledgements

MVK thanks the support of FAPESP (Processes #2014/01332-0 and #2021/06866-6) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (301436/2018-5). The first author is supported by a PhD scholarship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (142149/2018-7). We are also grateful to the four anonymous reviewers, whose helpful and positive feedback has been incorporated into a revised text. This paper is a contribution of NP-BioMar, USP.

Funding

This study was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (grants #2014/01332-0 and #2021/06866-6 to MVK) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant #301436/2018–5 to MVK and grant #142149/2018-7 to CVF).

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Authors and Affiliations

  1. Centre for Marine Biology, University of Sao Paulo, Sao Sebastiao, 11612-109, Brazil

    C. F. Vaga, I. G. L. Seiblitz, K. C. C. Capel & M. V. Kitahara

  2. Graduate Program in Zoology, Department of Zoology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, 05508-090, Brazil

    C. F. Vaga & I. G. L. Seiblitz

  3. Department of Marine Science, Federal University of Sao Paulo, Santos, 11070-100, Brazil

    K. C. C. Capel

  4. Department of Invertebrate Zoology, Smithsonian Institution, National Museum of Natural History, MRC 163, PO Box 37012, Washington, DC, 20013-7012, USA

    M. V. Kitahara

Authors
  1. C. F. Vaga
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  2. I. G. L. Seiblitz
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  3. K. C. C. Capel
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  4. M. V. Kitahara
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Contributions

CFV and MVK conceived and designed research. CFV conducted experiments. CFV, IGLS, and KCCC analyzed data. All authors wrote and approved the manuscript.

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Correspondence to C. F. Vaga.

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Vaga, C.F., Seiblitz, I.G.L., Capel, K.C.C. et al. The mitochondrial genomes of Crispatotrochus rubescens and Crispatotrochus rugosus (Hexacorallia; Scleractinia): new insights on the phylogeny of the family Caryophylliidae. Mol Biol Rep 49, 12269–12273 (2022). https://doi.org/10.1007/s11033-022-08029-1

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