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Development and characterization of microsatellite markers in the small Indian mongoose (Urva auropunctata)

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Abstract

Background

The small Indian mongoose (Urva auropunctata) is one of the world’s worst invasive alien species and eradication programs are ongoing worldwide. The development of individual and sex identification markers will improve their management.

Methods and results

We searched for novel mongoose microsatellite markers using genome-wide screening and identified 115,265 tetra-nucleotide repeat loci. Of 96 loci tested, 17 were genotyped in 28 mongooses from the Okinawa population. The genetic diversity analysis showed that the average expected and observed heterozygosity and number of alleles were 0.55, 0.56, and 2.94, respectively. Of 17 loci, one deviated from Hardy–Weinberg equilibrium and six loci pairs were likely linked to each other. However, we succeed in identifying all individuals using all of the microsatellite loci. The novel sex identification markers worked successfully in a test using sex known samples.

Conclusion

Our novel microsatellite and sex identification markers should be useful in studies of individual identification and population genetics of the mongoose.

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Acknowledgements

We are grateful to Mr. Katsushi Nakata, Dr. Ryoji Fukuhara, Yambaru Mongoose Busters and Yambaru Wildlife Center of the Ministry of Environment, Japan for providing samples, and Dr. Gohta Kinoshita, Ms. Yu Endo and Mr. Shinta Gima for their technical advices and supports. Computations were partially performed on the NIG supercomputer at ROIS National Institute of Genetics.

Funding

This research was performed by the Environment Research and Technology Development Fund (JPMEERF20204006) of the Environmental Restoration and Conservation Agency of Japan.

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Authors and Affiliations

  1. Okinawa University, Naha, 902-8521, Japan

    Takuma Sato

  2. Faculty of Law, Economics and Management, Okinawa University, Naha, 902-8521, Japan

    Takamichi Jogahara

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  1. Takuma Sato
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  2. Takamichi Jogahara
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Contributions

TS and TJ contributed to the research idea and manuscript writing. TS contributed to experimental design and data analysis.

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Correspondence to Takamichi Jogahara.

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Sato, T., Jogahara, T. Development and characterization of microsatellite markers in the small Indian mongoose (Urva auropunctata). Mol Biol Rep 48, 7029–7034 (2021). https://doi.org/10.1007/s11033-021-06655-9

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