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Effect of valproic acid on miRNAs affecting histone deacetylase in a model of anaplastic thyroid cancer

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Abstract

Background

Thyroid cancer is the most common malignant tumor of the endocrine system seen in the thyroid gland. More than 90% of thyroid cancers comprise papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC). Although anaplastic thyroid carcinoma (ATC) accounts for less than 2% of thyroid cancer. But patients’ lifespan after diagnosis is about 6 months. Surgical interventions, radioactive iodine use, and chemotherapy are not sufficient in the treatment of ATC, so alternative therapies are needed.

Methods and results

The WST-1 assay test was performed to evaluate the anti-proliferative effects of Valproic acid (VPA). Also, the effect of VPA on miRNAs affecting histone deacetylase was determined by Quantitative RT-PCR. In the SW1736 cell line, IC50 dose for VPA was found 1.6 mg/ml. In our study, the level of oncogenic genes expression in cells treated with VPA, including miR-184, miR-222-5p, miR-124-3p, and miR-328-3p, decreased. Also, the expression of tumor inhibitory genes including miR-323-5p, miR-182-5p, miR-138-5p, miR-217, miR-15a-5p, miR-29b-3p, miR-324-5p and miR-101-5p increased significantly.

Conclusions

VPA can ad-just countless gene expression patterns, including microRNAs (miRNAs), by targeting histone deacetylase (HDAC). However, further studies are required for more accurate results.

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Data availability

All data obtained in the study can be accessed if desired.

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Authors and Affiliations

  1. Department of Medical Biology, Ege University Medical School, Izmir, Turkey

    Nur Selvi Gunel, Nihal Birden, Cansu Caliskan Kurt, Bakiye Goker Bagca, Behrouz Shademan, Fatma Sogutlu, Neslihan Pinar Ozates & Cigir Biray Avci

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  1. Nur Selvi Gunel
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Contributions

NG: Writing—original draft. NB, FS, and NO: Methodology. CK: Methodology, data curation. BB: Data curation, methodology. CA: Conceptualization, writing—review & editing. BS: Conceptualization, writing—original draft.

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Correspondence to Cigir Biray Avci.

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Gunel, N.S., Birden, N., Kurt, C.C. et al. Effect of valproic acid on miRNAs affecting histone deacetylase in a model of anaplastic thyroid cancer. Mol Biol Rep 48, 6085–6091 (2021). https://doi.org/10.1007/s11033-021-06616-2

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  • DOI: https://doi.org/10.1007/s11033-021-06616-2

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