Abstract
Traditional Chinese medicine has been proven to be effective in treating human diseases according to a long-term observation for more than 2000 years. However, the precise molecular mechanisms of a majority of the medications are still largely unknown. Deer antler has been clinically used as an effective animal medication in traditional Chinese medicine for many centuries. Previous studies have demonstrated that antler extracts play crucial roles in promoting bone and cartilage development, growth and repair. However, the underlying molecular mechanism remains to be elucidated. In the present study, we applied isobaric tags for relative and absolute quantitation (iTRAQ) technology and a systematic bioinformatics analysis accompanied with validation method to obtain a full spectrum of the serum protein profiles under deer antler extract treatment. We identified a complex interaction network formed by the positive regulation of Tropomyosins (Tpm1, 2 and 4), WD repeat-containing protein 1 (Wdr1), Alpha-actinin-1 (Actn1) and Destrin (Dstn) and the negative regulation of Alpha-2-macroglobulin (A2m), Serine protease inhibitor A3 N (Serpina3n) and Apolipoproteins (Apoh and Apof), which coordinately interact with multiple proteins and signaling pathways. Our results suggest that the therapeutic effects of deer antler extract on treating bone diseases might achieved though the regulation of bone formation and remodeling by controlling a series of serum proteins and signaling pathways that were essential for osteoblast and osteoclast activities. Thus, this study has greatly deepened the current knowledge about the molecular mechanism of therapeutic effects of deer antler extract on bone diseases such as osteoporosis.
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Acknowledgements
This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFC1706605), the TCM Clinical Research Centre for Bone diseases of Jilin Province (Grant No. 20180623048TC) and the Science and Technology Development Project of Jilin Province (Grant No. 20170520044JH).
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All procedures were performed in accordance with the guidelines of the Institutional Animal Ethics Committee of Changchun University of Chinese Medicine (No. ccucm-2017-0015).
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Yao, B., Gao, H., Liu, J. et al. Identification of potential therapeutic targets of deer antler extract on bone regulation based on serum proteomic analysis. Mol Biol Rep 46, 4861–4872 (2019). https://doi.org/10.1007/s11033-019-04934-0
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DOI: https://doi.org/10.1007/s11033-019-04934-0