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Isoflavones prevent oxidative stress and inhibit the activity of the enzyme monoamine oxidase in vitro

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Abstract

Oxidative stress occurs due to an imbalance between antioxidant defenses and pro-oxidant agents in brain. This condition has been associated to the pathogenesis of several brain diseases; therefore, increasing the use of compounds that exert antioxidant activity. Thus, the objective of this study was to evaluate, in vitro, the effect of isoflavones in: (1) lipid peroxidation, catalase activity and thiol groups in the presence of pro-oxidants: sodium nitroprusside or Fe2+/EDTA complex in rat brain homogenates; (2) the activity of the enzyme monoamine oxidase (MAO). As a result, the isoflavones reduced lipid peroxidation in a manner dependent on the concentration and protected against the reduction of catalase activity as well as the induced thiol oxidation in brain tissue. In addition, isoflavones inhibited MAO activity (MAO-A and MAO-B). Taken together, our results showed that isoflavones avoided oxidative stress and decreased the MAO activity, suggesting a promissory use in the treatment of neurodegenerative diseases.

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Acknowledgements

We acknowledge fellowships from CNPq (R.F.) and CAPES (L.F.S., A.B., C.M.F, L.R.P).

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001, and CAPES/PROEX (23038.005848/2018-31; support number: 0737/2018). Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul – FAPERGS/Brazil (2080–2551/13-5-PqG-001/2013) and Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq/Brazil (475210/2013-1).

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Authors and Affiliations

  1. Curso de Biomedicina, Universidade Franciscana (UFN), Santa Maria, Rio Grande do Sul, Brazil

    Izaviany da Silva Schmitz & Larissa Finger Schaffer

  2. Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil

    Alcindo Busanello & Roselei Fachinetto

  3. Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil

    Catiuscia Molz de Freitas & Roselei Fachinetto

  4. Mestrado em Ciências da Saúde e da Vida, Universidade Franciscana (UFN), Santa Maria, Rio Grande do Sul, 97010-032, Brazil

    Luis Ricardo Peroza

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  1. Izaviany da Silva Schmitz
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Correspondence to Luis Ricardo Peroza.

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da Silva Schmitz, I., Schaffer, L.F., Busanello, A. et al. Isoflavones prevent oxidative stress and inhibit the activity of the enzyme monoamine oxidase in vitro. Mol Biol Rep 46, 2285–2292 (2019). https://doi.org/10.1007/s11033-019-04684-z

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