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Fibroblast growth factor 10 (FGF10) promotes the adipogenesis of intramuscular preadipocytes in goat

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Abstract

Fibroblast growth factor 10 (FGF10) is an adipokine that is found to participate in the regulation of adipogenesis. However, its function remains to be elucidated in intramuscular fat (IMF) deposition of goat. The purpose of this study was to explore the role of FGF10 in goat IMF deposition. Here, we investigated the expression of FGF10 in goat intramuscular adipocytes inducing 0, 2, 4, 6 and 8 days. Effect of FGF10 on adipogenesis was investigated by gaining and losing function of FGF10 in vitro. And then, we examined several lipid metabolism-related genes, including peroxisome proliferator activated receptor γ (PPARγ), sterol regulatory element binding protein 1 (SREBP1), preadipocyte factor-1 (Pref-1), CCAAT/enhancer binding protein-α (C/EBPα) and CCAAT/enhancer binding protein-β (C/EBPβ), as well as, Krüppel-like factor (KLF) family. We found that the sharp expression of FGF10 appeared at 2 days. Overexpression of FGF10 mediated by adenovirus promotes lipid accumulation, accompanied by up-regulating of LPL and C/EBPα with the down-regulating of C/EBPβ. Conversely, the expression of LPL, C/EBPα and SREBP1 was significantly decreased by the siRNAs of FGF10. Meanwhile, we showed that FGF10 regulated the expression of many KLFs members and interacted synergistically or antagonistically with them. Thus, our results demonstrated a key role of FGF10 as a positively factor in the regulation of adipogenic differentiation of intramuscular preadipocyte in goat.

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Acknowledgements

This research was supported by the Science and Technology Support Program of Sichuan Province (2016NYZ0045), NationalNatural Science Foundation of China (31672395 and 31601921), and Fundamental Research Funds for the Central Universities, Southwest Minzu University (2017NGJPY06). We are grateful to Dr. Bai Wenlin for proofreading this manuscript and modify the statement critically.

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Author notes

  1. Qing Xu and Sen Lin have contributed equally to this work.

Authors and Affiliations

  1. School of Life Science and Technology, Southwest Minzu University, Chengdu, 610041, China

    Qing Xu, Sen Lin, Yong Wang, Jiangjiang Zhu & Yaqiu Lin

  2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation, Southwest Minzu University, Chengdu, 610041, Sichuan, China

    Sen Lin, Yong Wang & Jiangjiang Zhu

Authors
  1. Qing Xu
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  2. Sen Lin
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  3. Yong Wang
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  5. Yaqiu Lin
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Correspondence to Yong Wang or Yaqiu Lin.

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Xu, Q., Lin, S., Wang, Y. et al. Fibroblast growth factor 10 (FGF10) promotes the adipogenesis of intramuscular preadipocytes in goat. Mol Biol Rep 45, 1881–1888 (2018). https://doi.org/10.1007/s11033-018-4334-1

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  • DOI: https://doi.org/10.1007/s11033-018-4334-1

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