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A l-proline/O2 biofuel cell using l-proline dehydrogenase (LPDH) from Aeropyrum pernix

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Abstract

To utilize amino acids from food waste as an energy source, l-proline/O2 biofuel cell was constructed using a stable enzyme from hyperthermophilic archaeon for long-term operation. On the anode, the electrocatalytic oxidation of l-proline by l-proline dehydrogenase from Aeropyrum pernix was observed in the presence of ferrocenecarboxylic acid as mediator. On the cathode, electrocatalytic oxygen reduction was detected. Ketjenblack modification of carbon cloth substrate increased the current density due to increased laccase loading and enhanced electron transfer reaction. The biofuel cell using these electrodes achieved a current density of 6.00 µA/cm2. We successfully constructed the first biofuel cell that generates power from l-proline.

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Authors and Affiliations

  1. Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan

    Aina Tonooka, Tomohiro Komatsu, Hiroaki Sakamoto & Shin-ichiro Suye

  2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui, Japan

    Shino Tanaka, Takenori Satomura & Shin-ichiro Suye

Authors
  1. Aina Tonooka
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  2. Tomohiro Komatsu
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  3. Shino Tanaka
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  4. Hiroaki Sakamoto
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  5. Takenori Satomura
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  6. Shin-ichiro Suye
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Corresponding authors

Correspondence to Hiroaki Sakamoto or Shin-ichiro Suye.

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The authors declare that they have no conflict of interest.

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Tonooka, A., Komatsu, T., Tanaka, S. et al. A l-proline/O2 biofuel cell using l-proline dehydrogenase (LPDH) from Aeropyrum pernix. Mol Biol Rep 45, 1821–1825 (2018). https://doi.org/10.1007/s11033-018-4328-z

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  • DOI: https://doi.org/10.1007/s11033-018-4328-z

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