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Hypoxic stress: impact on the modulation of TLR2, TLR4, NOD1 and NOD2 receptor and their down-stream signalling genes expression in catla (Catla catla)

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Abstract

The damage-associated molecular patterns (DAMPs) released from the damaged tissue/cells are recently reported as endogenous ligands to activate toll-like receptors (TLRs) and nucleotide binding and oligomerization domain (NOD) receptors signaling pathways. In the aquatic environment, reduction in dissolved oxygen (DO) concentration causes hypoxic stress resulting in tissue damage and patho-biological changes in fish. We envisaged the critical role of TLR and NOD receptors in recognizing DAMPs as endogenous ligands during hypoxic stress in fish. Catla (Catla catla) fingerlings (avg. wt ~56 g) was exposed to hypoxic stress (DO: 1–3 mg/L) for 1 and 24 h. After the designated time course, total RNA was extracted from gill, liver, kidney and blood, and modulation of TLRs (TLR2 and TLR4), NOD (NOD1 and NOD2) receptors, MyD88 (myeloid differentiation primary response gene 88), RICK (receptor interacting serine-threonine protein kinase-2), interleukin (IL)-6, IL-8 and IL-10 gene expression were analyzed by quantitative reverse transcriptase PCR assay. Significant (p < 0.05) up-regulation of some DAMPs {high-mobility group box 1 and heat shock protein-70}, TLRs and NOD receptors genes expressions were observed in the hypoxic fish tissues as compared to the control. Further investigation revealed inductive expression of MyD88, RICK, IL-6, IL-8 and IL-10 genes in the TLRs and NODs activated tissues of the hypoxic fish. These data together may suggest the important role of TLRs and NOD receptors signaling pathway in sterile inflammation and pathobiology of fish in hypoxic stress, and warrant further study to investigate the role of TLR and NOD receptors in abiotic stress management in aquaculture.

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Acknowledgments

This work was supported by the Grant of National Agricultural Science Fund (NASF), Indian Council of Agricultural Research (ICAR), Govt. of India (Project code AS-2001 and BS-4003). The authors express their gratitude to the Director CIFA, for providing necessary facility, Dr A Bandyopadhyay former NC, NASF for his constructive criticism in project formulation, and Dr P. K. Agrawal, NC, NASF for help, suggestions and guidance in executing the project.

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    Authors and Affiliations

    1. Fish Health Management Division, ICAR-Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, Odisha, 751002, India

      Madhubanti Basu, Mahismita Paichha, Saswati S. Lenka & Mrinal Samanta

    2. Aqua Research Lab, Department of Zoology, University of Delhi, Delhi, 110007, India

      Rina Chakrabarty

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    1. Madhubanti Basu
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    Correspondence to Mrinal Samanta.

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    Madhubanti Basu and Mahismita Paichha these authors have contributed equally.

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    Basu, M., Paichha, M., Lenka, S.S. et al. Hypoxic stress: impact on the modulation of TLR2, TLR4, NOD1 and NOD2 receptor and their down-stream signalling genes expression in catla (Catla catla). Mol Biol Rep 43, 1–9 (2016). https://doi.org/10.1007/s11033-015-3932-4

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    • DOI: https://doi.org/10.1007/s11033-015-3932-4

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