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Induction of toll-like receptor (TLR) 2, and MyD88-dependent TLR- signaling in response to ligand stimulation and bacterial infections in the Indian major carp, mrigal (Cirrhinus mrigala)

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Abstract

Toll-like receptor 2 (TLR2) is a member of TLR family. It recognizes a wide range of bacteria and their products, and is involved in inducing innate immune responses. In this article, we reported inductive expression of TLR2 and myeloid differentiation primary response gene 88 (MyD88)-dependent signaling in the Indian major carp, mrigal (Cirrhinus mrigala) which is highly commercially important fish species in the Indian subcontinent. Ontogeny analysis of TLR2, MyD88 and TRAF6 (TNF receptor associated factor 6) genes by quantitative real-time PCR (qRT-PCR) revealed constitutive expression of these genes in all embryonic developmental stages, indicating their involvement in embryonic innate immune defense system in fish. Tissue specific expression analysis of these genes by qRT-PCR showed their wide distribution in various organs and tissues. Highest expression of TLR2 was in gill, MyD88 in liver and TRAF6 was in kidney. Inductive expression of TLR2, MyD88 and TRAF6 genes were observed following peptidoglycan (PGN)-treatment, and Streptococcus uberis and Aeromonas hydrophila infections. Expression of interleukin (IL)-8 and TNF-α in various organs were significantly enhanced by PGN-treatment and bacterial infections, and were closely associated with TLR2 induction. These findings together highlighted the contribution of TLR2 in augmenting innate immunity in fish, and indicated it’s important role in immune surveillance of various organs during pathogenic invasion. This study will enrich the information in understanding the innate immune mechanism in fish, and will be helpful in developing preventive measures against infectious diseases in fish.

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Acknowledgments

This work was financially supported by the grant of National Agricultural Innovation Project (NAIP), Indian Council of Agricultural Research (ICAR) (Project code C4-C30018). We express our sincere gratitude to the Director, CIFA for providing necessary facility to carry out the work. We thank Dr. P. Routray, Senior Scientist, Aquaculture Production and Environment Division, CIFA, for hatchery facility and various suggestions.

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Authors and Affiliations

  1. Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, 751002, Orissa, India

    Madhubanti Basu, Banikalyan Swain, Bikash R. Sahoo, Nikhil K. Maiti & Mrinal Samanta

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  1. Madhubanti Basu
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  2. Banikalyan Swain
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  3. Bikash R. Sahoo
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  4. Nikhil K. Maiti
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  5. Mrinal Samanta
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Correspondence to Mrinal Samanta.

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Basu, M., Swain, B., Sahoo, B.R. et al. Induction of toll-like receptor (TLR) 2, and MyD88-dependent TLR- signaling in response to ligand stimulation and bacterial infections in the Indian major carp, mrigal (Cirrhinus mrigala) . Mol Biol Rep 39, 6015–6028 (2012). https://doi.org/10.1007/s11033-011-1415-9

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  • DOI: https://doi.org/10.1007/s11033-011-1415-9

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