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Comparative proteomic analysis of seedling leaves of cold-tolerant and -sensitive spring soybean cultivars

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Abstract

Cold stress adversely affects the growth and development of seedling of spring soybean. Revealing responses in seedling to cold stress at proteomic level will help us to breed cold-tolerant spring soybean cultivars. In this study, to understand the responses, a proteomic analysis on the leaves of seedlings of one cold-tolerant soybean cultivar and one cold-sensitive soybean cultivar at 5 °C for different times (12 and 24 h) was performed, with some proteomic results being further validated by physiological and biochemical analysis. Our results showed that 57 protein spots were found to be significantly changed in abundance and identified by MALDI-TOF/TOF MS. All the identified proteins were found to be involved in 13 metabolic pathways and cellular processes, including photosynthesis, protein folding and assembly, cell rescue and defense, cytoskeletal proteins, transcription and translation regulation, amino acid and nitrogen metabolism, protein degradation, storage proteins, signal transduction, carbohydrate metabolism, lipid metabolism, energy metabolism, and unknown. Based on the majority of the identified cold-responsive proteins, the effect of cold stress on seedling leaves of the two spring soybean cultivars was discussed. The reason that soybean cv. Guliqing is more cold-tolerant than soybean cv. Nannong 513 was due to its more protein, lipid and polyamine biosynthesis, more effective sulfur-containing metabolite recycling, and higher photosynthetic rate, as well as less ROS production and lower protein proteolysis and energy depletion under cold stress. Such a result will provide more insights into cold stress responses and for further dissection of cold tolerance mechanisms in spring soybean.

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Abbreviations

DAF-2DA:

4,5-Diaminofluorescein diacetate

MOPS:

3-(Nmorpholino) propanesulfonic acid

NO:

Nitric oxide

PPP:

Pentose phosphate pathway

PUT:

Putrescine

ROS:

Reactive oxygen species

SPD:

Spermidine

SPM:

Spermine

Unipro:

Unique protein

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Acknowledgments

We gratefully acknowledge the partial financial support from the projects supported by the National Natural Science Foundation of China (30971840, 31171572, 31101212, 91117006), the National Key Basic Research Program of China (2010CB134403), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20100097110030, 20120097110025), the Science and Technology Development Foundation of Shanghai Agricultural Academy, China (2000-04-06-3), the Agriculture Science and Technology Fund of the Ministry of Science and Technology, China (02EFN216901241, 04EFN213100094), the Shanghai Committee of Science and Technology, China (093919N1400, 12391900900), and the Priority Academic Program Development of Jiangsu Higher Education Institution for this research.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Xin Tian, Huaping Duan, Xiaoling He & Hao Ma

  2. College of Agriculture, Hebei University of Engineering, Handan, 056038, Hebei, People’s Republic of China

    Ying Liu

  3. Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, 410128, Hunan, People’s Republic of China

    Zhigang Huang, Jianhua Tong & Langtao Xiao

  4. Animal and Plant Introduction and Research Center, Shanghai Agricultural Academy, Shanghai, 201106, People’s Republic of China

    Weihong Gu

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  1. Xin Tian
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  2. Ying Liu
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  3. Zhigang Huang
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  4. Huaping Duan
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  8. Hao Ma
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Correspondence to Hao Ma or Langtao Xiao.

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Xin Tian, Ying Liu, Zhigang Huang have contributed equally to this work.

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11033_2014_3803_MOESM1_ESM.docx

Supplementary 1 2-DE image analysis of seedling leaf proteome of the two soybean cvs Guliqing and Nannong 513 under cold stress and control. (DOCX 2248 kb)

Supplementary 2 MALDI-TOF/TOF MS (RAR 3943 kb)

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Tian, X., Liu, Y., Huang, Z. et al. Comparative proteomic analysis of seedling leaves of cold-tolerant and -sensitive spring soybean cultivars. Mol Biol Rep 42, 581–601 (2015). https://doi.org/10.1007/s11033-014-3803-4

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