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Transcriptome analysis of the Bombyx mori fat body after constant high temperature treatment shows differences between the sexes

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Abstract

Ambient temperature plays a large role in insect growth, development and even their distribution. The elucidation of the associated molecular mechanism that underlies the effect of constant high temperature will enables us to further understand the stress responses. We constructed four digital gene expression libraries from the fat body of female and male Bombyx mori. Differential gene expression was analyzed after constant high temperature treatment. The results showed that there were significant changes to the gene expression in the fat body after heat treatment, especially in binding, catalytic, cellular and metabolic processes. Constant high temperature may induce more traditional cryoprotectants, such as glycerol, glycogen, sorbitol and lipids, to protect cells from damage, and induce heat oxidative stress in conjunction with the heat shock proteins. The data also indicated a difference between males and females. The heat shock protein-related genes were up-regulated in both sexes but the expression of Hsp25.4 and DnaJ5 were down-regulated in the male fat body of B. mori. This is the first report of such a result. Constant high temperature also affected the expression of other functional genes and differences were observed between male and female fat bodies in the expression of RPS2, RPL37A and MREL. These findings provide abundant data on the effect of high temperature on insects at the molecular level. The data will also be beneficial to the study of differences between the sexes, manifested in variations in gene expression under high temperature.

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Acknowledgments

The authors gratefully acknowledge financial support from the earmarked fund for China Agriculture Research System (CARS; Grant No. CARS-22), the National High-Tech R&D Program of China (863 Program; Grant No. 2011AA100306) and Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. Department of Applied Biology, School of Biology and Basic Medical Sciences, Medical College of Soochow University, Suzhou, 215123, China

    Hua Wang, Yan Fang, Lipeng Wang, Wenjuan Zhu, Haipeng Ji, Haiying Wang, Shiqing Xu & Yanghu Sima

  2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 215123, China

    Shiqing Xu & Yanghu Sima

  3. Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin, 300071, China

    Hua Wang

  4. Department of Immunology, Nankai University School of Medicine, Tianjin, 300071, China

    Yan Fang

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  1. Hua Wang
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Correspondence to Yanghu Sima.

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Supplementary file 1 Gene-specific primers for this study. (DOC 45 kb)

11033_2014_3481_MOESM2_ESM.tif

Supplementary file 2 Gene-pathway network graph of the male B. mori. The relativity of pathways, based on the significance of input gene enrichment in the pathway. We used all the Up/Down-regulated gene data in the MAS software. (TIFF 5870 kb)

Supplementary file 3 Gene-pathway network graph of the female B. mori. Similar to the male. (TIFF 9004 kb)

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Wang, H., Fang, Y., Wang, L. et al. Transcriptome analysis of the Bombyx mori fat body after constant high temperature treatment shows differences between the sexes. Mol Biol Rep 41, 6039–6049 (2014). https://doi.org/10.1007/s11033-014-3481-2

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