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Gene expression profiles of arabidopsis under the stress of methyl viologen: a microarray analysis

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Abstract

Methyl viologen (MV) is the main ingredient of Paraquat. It is little known about how plants respond to this compound. To understand the mode of MV action and molecular mechanism of plant response, we performed experiments of microarray on Arabidopsis. In MV treated seedling, approximately 6 % genes were altered at mRNA levels, including 818 genes increased, whereas 1,440 genes decreased. Studies of these genes expression patterns provided some new information on the reaction process of plant after the treatment with MV. These included signaling molecules for MV response and reactive oxygen species formation, enzymes required for secondary metabolism and, cell wall maintenance and strategy of photostasis balance. The expression kinetics of the genes induced by MV will provides useful information for the abiotic stress defense mechanism in plants.

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Acknowledgments

The research was supported by the Key Project Fund of the Shanghai Municipal Committee of Agriculture (No. 2011-1-8) and International Scientific and Technological Cooperation (2010DFA62320, 11230705900) and National Natural Science Foundation (31071486).The Key Project Fund of Science and Technology Committee of the Shanghai Minhang Municipality (2012MH059).Young Foundation of Shanghai Academy of Agricultural Science (2012-16, 2010-14). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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  1. Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, 201106, People’s Republic of China

    Hong-Juan Han, Ri-He Peng, Bo Zhu, Xiao-Yan Fu, Wei Zhao, Biao Shi & Quan-Hong Yao

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  1. Hong-Juan Han
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  2. Ri-He Peng
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  3. Bo Zhu
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Correspondence to Biao Shi or Quan-Hong Yao.

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Supplementary material Table 1 Sequences for all primer sets used for RT-PCR (DOC 32 kb)

11033_2014_3396_MOESM2_ESM.doc

Supplementary material Table 2 Significant genes up and down-regulated (≥ 2.0 folds and ≤ 10 % cv) in response to Methyl Viologen (DOC 2543 kb)

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Han, HJ., Peng, RH., Zhu, B. et al. Gene expression profiles of arabidopsis under the stress of methyl viologen: a microarray analysis. Mol Biol Rep 41, 7089–7102 (2014). https://doi.org/10.1007/s11033-014-3396-y

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