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TGF-beta signaling-dependent alleviation of dextran sulfate sodium-induced colitis by mesenchymal stem cell transplantation

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Abstract

Alleviation of dextran sulfate sodium (DSS)-induced colitis was shown after by transplantation of bone marrow derived cells in mice. Nevertheless, the underlying mechanism remains elusive. In the present study, we transplanted primary mouse mesenchymal stem cells (MSC) into isogeneic mice with DSS-induced colitis. We found that MSC transplantation significantly alleviated the DSS-induced colitis. Inhibition of transforming growth factor beta (TGF-beta) signaling abrogated the therapeutic effect of MSC transplantation on DSS-colitis, suggesting a TGF-beta signaling-dependent manner. Moreover, MSC transplantation seemed to induce M2 macrophage polarization, which appeared to be the major source of TGF-beta in this model. Our data thus demonstrate that MSC transplantation may activate TGF-beta signaling pathways to promote the recovery of DSS-colitis.

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Abbreviations

MSC:

Mesenchymal stem cell

DSS:

Dextran sulfate sodium

TGF-beta:

Transforming growth factor beta

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (81300418), from China Postdoctoral Science Foundation funded project (2012M511591, 2013T60708) and The Youth Innovation Fund of The First Affiliated Hospital of Zhengzhou University.

Conflict of interest

The authors have declared that no competing interests exist.

Author information

Authors and Affiliations

  1. Department of Hematology, The First Affiliated Hospital of Zhengzhou University, 1 Construction East Road, Zhengzhou, 450052, Henan Province, China

    Chong Wang, Ling Sun & Yanfang Liu

  2. Nursing College of Zhengzhou University, 3 Construction East Road, Zhengzhou, 450052, Henan Province, China

    Jing Chen

Authors
  1. Chong Wang
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  2. Jing Chen
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  3. Ling Sun
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  4. Yanfang Liu
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Correspondence to Yanfang Liu.

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Wang, C., Chen, J., Sun, L. et al. TGF-beta signaling-dependent alleviation of dextran sulfate sodium-induced colitis by mesenchymal stem cell transplantation. Mol Biol Rep 41, 4977–4983 (2014). https://doi.org/10.1007/s11033-014-3364-6

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  • DOI: https://doi.org/10.1007/s11033-014-3364-6

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