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Interferon gamma and Interleukin 10 polymorphisms in Brazilian patients with systemic lupus erythematosus

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Abstract

The pathogenesis of systemic lupus erythematosus (SLE) is complex, with several susceptibility genes and environmental factors involved in its development and clinical manifestation. Currently, there is a great amount of interest in the identification of biomarkers, as cytokines, that can quantify the susceptibility of SLE, the risk of future organ involvement, and association of their changes with disease activity. To investigate the associations between polymorphisms in the gene of Interferon gamma (IFN-γ) and in the promoter of the Interleukin-10 (IL-10) gene and SLE. The polymorphisms +874 T/A (rs2430561) in the IFN-γ gene and −1082G/A (rs1800896) in the IL-10 promoter were determined in 99 SLE patients and 100 healthy controls among women Brazilian using the refractory mutation system polymerase chain reaction method. Disease activity was assessed using the SLE activity index. There were significant differences in the distribution of the genotype T/A in IFN-γ gene polymorphism (+874) (χ 2 = 7.168; P = 0.0074) and the genotype G/A in IL-10 promoter polymorphism (−1082) (χ 2 = 4.654; P = 0.0310) between the SLE and control groups. However, no association was observed between clinical features and the polymorphisms studied. This study presents preliminary evidence for association between IL-10 and IFN-γ polymorphism and SLE susceptibility, but not with clinical features in a Northeast population from Brazil.

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Acknowledgments

We thank the patients and their families, whose collaboration and understanding have made this work possible. This study was supported by FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

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Authors and Affiliations

  1. Campus Garanhuns of Federal Institute of Pernambuco (Campus Garanhuns/IFPE), Garanhuns, Brazil

    Hildson Dornelas Angelo da Silva

  2. Universidade Federal de Pernambuco (PPGG/UFPE), Teresina, Brazil

    Hildson Dornelas Angelo da Silva & Helker Albuquerque da Silva

  3. Laboratory of Biochemical Genetics and DNA Sequencing Professora Tânia Falcão, Universidade Federal Rural de Pernambuco (Genoma/UFRPE), Recife, Brazil

    Alex Paulino da Silva

  4. Departament of Nutrition, Universidade de Pernambuco (UPE), Santo Amaro, Brazil

    Helker Albuquerque da Silva

  5. Departament of Clinical Medicine, Faculty of Medicine, Universidade Federal de Pernambuco (UFPE), Cidade Universitari, Brazil

    Nadja Maria Jorge Asano

  6. Departament of Biology, Universidade Federal Rural de Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, 52171-900, PE, Brazil

    Maria de Mascena Diniz Maia & Paulo Roberto Eleutério de Souza

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  1. Hildson Dornelas Angelo da Silva
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Correspondence to Paulo Roberto Eleutério de Souza.

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da Silva, H.D.A., da Silva, A.P., da Silva, H.A. et al. Interferon gamma and Interleukin 10 polymorphisms in Brazilian patients with systemic lupus erythematosus. Mol Biol Rep 41, 2493–2500 (2014). https://doi.org/10.1007/s11033-014-3106-9

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  • DOI: https://doi.org/10.1007/s11033-014-3106-9

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