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BcPMI2, isolated from non-heading Chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco

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Abstract

Phosphomannose isomerase (PMI) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (AsA) biosynthesis in plants. To clarify the physiological roles of PMI in AsA biosynthesis, the cDNA sequence of PMI was cloned from non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and overexpressed in tobacco transformed with Agrobacterium tumefaciens. The AsA and soluble sugar contents were lower in 35S::BcPMI2 tobacco than in wild-type tobacco. However, the AsA level in BcPMI2-overexpressing plants under stress was significantly increased. The T1 seed germination rate of transgenic plants was higher than that of wild-type plants under NaCl or H2O2 treatment. Meanwhile, transgenic plants showed higher tolerance than wild-type plants. This finding implied that BcPMI2 overexpression improved AsA biosynthetic capability and accumulation, and evidently enhanced tolerance to oxidative and salt stress, although the AsA level was lower in transgenic tobacco than in wild-type tobacco under normal condition.

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Abbreviations

AsA:

l-Ascorbic acid

Fru-6P:

Fructose-6-phosphate

GDH:

l-Galactose dehydrogenase

GGP:

GDP-l-galactose phosphorylase

GGT:

GDP-l-galactose transferase

GLDH:

l-Galactono-1,4-lactone dehydrogenase

GME:

GDP-mannose 3′,5′-epimerase

GMP:

GDP-d-mannose pyrophosphorylase

Man-6P:

Mannose-6-phosphate

PMI:

Phosphomannose isomerase

PMM:

Phosphomannomutase

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Acknowledgments

This work was supported by 973 Program (2009CB119001-04), Science & Technology Pillar Program of Jiangsu Province (BE2012325) and SRT Project of Nanjing Agricultural University (1114A11) and Jiangsu Province (JSS1112), China.

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Authors and Affiliations

  1. Horticultural Department, Nanjing Agricultural University, Nanjing, 210095, China

    Xuehua Wang, Shuo Zhang, Die Hu, Xiaojun Zhao, Yan Li, Tongkun Liu, Jianjun Wang, Xilin Hou & Ying Li

  2. State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing, 210095, China

    Xuehua Wang, Shuo Zhang, Die Hu, Xiaojun Zhao, Yan Li, Tongkun Liu, Jianjun Wang, Xilin Hou & Ying Li

  3. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing, 210095, China

    Xuehua Wang, Shuo Zhang, Die Hu, Xiaojun Zhao, Yan Li, Tongkun Liu, Jianjun Wang, Xilin Hou & Ying Li

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  1. Xuehua Wang
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  2. Shuo Zhang
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Correspondence to Ying Li.

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Wang, X., Zhang, S., Hu, D. et al. BcPMI2, isolated from non-heading Chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco. Mol Biol Rep 41, 2207–2216 (2014). https://doi.org/10.1007/s11033-014-3072-2

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