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BcMF21 is important for pollen development and germination in Brassica campestris ssp. chinensis

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Abstract

Brassica campestris Male Fertility 21 (BcMF21) was previously isolated from the flower buds of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. Here, we used antisense RNA technology to knock down the expression level of BcMF21 in B. campestris and analyzed the phenotype of the transgenic plants. Alexander staining and scanning electron microscope revealed sterility and exine deformities in the mature pollen grains of BcMF21 antisense RNA transgenic plants. The germination furrow of the BcMF21 antisense RNA transgenic pollen was covered by lipid like materials. The pollen tubes burst and could not grow normally in vitro. Therefore, we presented here BcMF21 might be an important gene for pollen development and germination.

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Acknowledgments

This work was supported by the National Program on Key Basic Research Projects (No. 2012CB113900), Natural Science Foundation of China (No. 31071805) and the Key Sci-Technology Project of Zhejiang Province (No. 2010C12004).

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Authors and Affiliations

  1. Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China

    Jingjing Jiang, Youjian Yu, Heng Dong, Lina Yao, Zhixian Zhang & Jiashu Cao

  2. State Key Lab of Agrobiotechnology Shenzhen Base, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, 518057, China

    Jingjing Jiang

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  1. Jingjing Jiang
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  2. Youjian Yu
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  3. Heng Dong
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  6. Jiashu Cao
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Correspondence to Jiashu Cao.

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Jiang, J., Yu, Y., Dong, H. et al. BcMF21 is important for pollen development and germination in Brassica campestris ssp. chinensis . Mol Biol Rep 41, 537–544 (2014). https://doi.org/10.1007/s11033-013-2889-4

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  • DOI: https://doi.org/10.1007/s11033-013-2889-4

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