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Tumor suppressor ING4 overexpression contributes to proliferation and invasion inhibition in gastric carcinoma by suppressing the NF-κB signaling pathway

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Abstract

There is growing evidence that inhibitor of growth 4 (ING4) plays a pivotal role in development and progression of multiple different tumors; however, its precise function in gastric carcinoma remains to be elucidated. In the present study, we investigated ING4 level in gastric carcinoma tissues and cells, and preliminarily elucidated the role of ING4 in the proliferation and invasion of gastric carcinoma. The results demonstrated that expressions of ING4 mRNA and protein in gastric carcinoma tissues and cells were significantly lower than those in normal tissues and cells (P < 0.05). ING4 level in gastric carcinoma cells stably expressing ING4 was markedly higher than those in untreated group and empty vector pcDNA3.1 group (P < 0.05). Elevated ING4 level resulted in the inhibition of proliferation and invasion in three of gastric carcinoma cell lines MKN-28, SGC-7901 and MKN-45. Most notably, increased ING4 level evidently evoked the down-regulation of p65, p-IκBα, MMP-9 and uPA proteins and the up-regulation of IκBα protein. Our results presented herein suggest that ING4 level elevation mediated proliferation and invasion inhibition may be tightly associated with the suppression of NF-κB signaling pathway.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 81071723 and 81272691).

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Authors and Affiliations

  1. Department of General Surgery, Huaihe Hospital of Henan University, No.8 Baogong Lake North Road, Kaifeng, 475000, Henan, People’s Republic of China

    Shijie Li, Jiangtao Chen, Changjiang Qin & Xuequn Ren

  2. Department of Pharmacology, Basic Medical College of Zhengzhou University, Zhengzhou, 450001, Henan, People’s Republic of China

    Tianli Fan

  3. Department of Bioengineering of Zhengzhou University, Zhengzhou, 450001, Henan, People’s Republic of China

    Hongtao Liu

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  1. Shijie Li
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Correspondence to Xuequn Ren.

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Shijie Li, Tianli Fan and Hongtao Liu have contributed equally to this study.

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Li, S., Fan, T., Liu, H. et al. Tumor suppressor ING4 overexpression contributes to proliferation and invasion inhibition in gastric carcinoma by suppressing the NF-κB signaling pathway. Mol Biol Rep 40, 5723–5732 (2013). https://doi.org/10.1007/s11033-013-2675-3

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  • DOI: https://doi.org/10.1007/s11033-013-2675-3

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