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Genetic diversity of MYH 3 gene associated with growth and carcass traits in Chinese Qinchuan cattle

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Abstract

MYH3, whose function is to convert chemical energy to mechanical energy through ATP hydrolysis, is mainly expressed in skeletal muscle at various stages and is indispensable in the procedure of development of skeletal muscle and heart. In the study, genetic variations and genotypes of MYH 3 gene in a total of 365 Qinchuan cattles were analyzed by polymerase chain reaction-restriction fragment length polymorphism, as well as verified the effect on growth and carcass traits. After PCR products were digested by restriction enzymes, eight SNPs were identified and individuals were genotyped. It showed that the SNPs at nucleotides were all in low linkage disequilibrium, therefore no dominated haplotype was found in the population. The result of statistic analysis indicated seven SNPs were significantly associated with growth and carcass traits (P < 0.05, N = 365) except locus G13791A. To sum up, the result of the study proved that polymorphisms in MYH3 gene are associated with the growth performance of Chinese Qinchuan cattle, so the variations of the gene could be used as possible molecular assisted-makers in the beef cattle breeding program and management.

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Abbreviations

PCR-RFLP:

Polymerase chain reaction-restriction fragment length polymorphism

SNPs:

Single nucleotide polymorphisms

MyHC:

Myosin heavy chain

MYH3:

Myosin heavy chain 3

LD:

Linkage disequilibrium

He:

Heterozygosity

Ho:

Homozygosity

PIC:

Polymorphism information content

FSS:

Freeman–Sheldon syndrome

SHS:

Sheldon–Hall syndrome

CK:

Creatine phosphokinase

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Acknowledgments

Research supported by the National 12th ‘‘Five-Year’’ National Science and Technology Key Project (No.2011AA100307), National 11th ‘‘Five-Year’’ National Science and Technology Key Project (No. 2008AA101010), ‘‘13115’’ Sci-Tech Innovation Program of Shaanxi Province (No.2008ZDKG-11).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Fubiao Niu, Lijun Wang, Xiaolin Liu, Hongliang Wang, Jing Yang, Yu Liu & Ling Chen

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  1. Fubiao Niu
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  2. Lijun Wang
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  3. Xiaolin Liu
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  4. Hongliang Wang
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  5. Jing Yang
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  6. Yu Liu
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  7. Ling Chen
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Corresponding author

Correspondence to Xiaolin Liu.

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Fubiao Niu and Lijun wang have equally contributed to this work.

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Niu, F., Wang, L., Liu, X. et al. Genetic diversity of MYH 3 gene associated with growth and carcass traits in Chinese Qinchuan cattle. Mol Biol Rep 40, 5635–5643 (2013). https://doi.org/10.1007/s11033-013-2665-5

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  • DOI: https://doi.org/10.1007/s11033-013-2665-5

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