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Relationship between gene polymorphisms of two cytokine genes (TNF-α and IL-6) and occurring of lung cancers in the ethnic group Han of China

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Abstract

Tumor necrosis factor (TNF) is a cytokine involved in inflammation and TNF-α might be synthesized ectopically in malignant tumors. Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. The present study is to investigate the relationship between genetic polymorphisms of the TNF-α and IL-6 genes and susceptibility to lung cancers in the ethnic group Han of North China. The genotypes in the −238G locus of TNF-α gene and the −572C locus of the IL-6 gene were determined by PCR-RFLP method in 138 patients with lung cancers and 138 healthy individuals. Software PHASE 1.0 was used to analyze the experimental data. The non-conditional logistic regression model was used to analyze the statistical association of genotypes and susceptibility in two groups adjusted by multiple factors. We found that the TNF-α and IL-6 polymorphisms may be a critical risk for the genetic susceptibility to lung cancers in the ethnic group Han of North China. SNP polymorphisms at the −238G locus of TNF-α gene and the −572C locus of the IL-6 gene were detected by the RFLP-PCR method. We found that high rates of single-base G-to-A alteration at the −238G locus of both alleles and high rates of single-base C-to-G alteration at the −572C locus of both alleles correlated with occurring of lung cancers. It is possible that the SNP markers at the −238G locus of TNF-α gene and the −572C locus of the IL-6 gene serve as biological markers of lung cancers upon further study in the future.

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Acknowledgments

This work is supported by Natural Science Foundation of Shandong Province, China (Grant No. 2005ZX04).

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Authors and Affiliations

  1. Shandong Provincial Qianfoshan Hospital, Affiliated to Shandong University, No. 16766 Jing Shi Road, LiXia District, Jinan, 250014, Shandong, China

    Jing Liang, Xiaolin Liu, Beibei Yin, Junjuan Xiao, Hairong Liu & Yan Li

  2. Shandong Center for Disease Control and Prevention, No. 16992 Jing Shi Road, LiXia District, Jinan, 250014, Shandong, China

    Zhenqiang Bi

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  1. Jing Liang
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  2. Xiaolin Liu
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  3. Zhenqiang Bi
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  7. Yan Li
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Correspondence to Yan Li.

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Liang, J., Liu, X., Bi, Z. et al. Relationship between gene polymorphisms of two cytokine genes (TNF-α and IL-6) and occurring of lung cancers in the ethnic group Han of China. Mol Biol Rep 40, 1541–1546 (2013). https://doi.org/10.1007/s11033-012-2199-2

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  • DOI: https://doi.org/10.1007/s11033-012-2199-2

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