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Characterization of grapevine microR164 and its target genes

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Abstract

MicroRNAs (miRNAs) are an extensive class of newly identified small RNAs that regulate gene expression at post-transcription level by mRNA cleavage or translation. In our study, we used qRT-PCR and found that Vv-miR164 is expression in grapevine leaves, stems, tendrils, inflorescences, flowers and fruits. In addition, two potential target genes for Vv-miR164 were also found and verified by PPM-RACE and RLM-RACE. The results not only maps the cleavage site of the target mRNA but allowed for detection the expression pattern of cleaved fragments that can indicate the regulatory function of this miRNA on its target genes. These target genes were explored by qRT-PCR where some exhibited different expression patterns from their corresponding miRNA, indicating the cleavage mode of the miRNA on its target genes. The efficient and powerful approach used in this study can help in further understanding of how miRNAs cleaved their target mRNAs. Results from this study prove the importance of Vv-miR164 in regulating development and growth of grapes, and adds to the existing knowledge of small RNA-mediated regulation in grapes.

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Acknowledgments

This work was supported by A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the NCET Program of China (Grant No. NCET-08-0796).

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  1. College of Horticulture, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Xin Sun, Nicholas Kibet Korir, Jian Han, Ling-Fei Shangguan, Emrul Kayesh, Xiang-Peng Leng & Jing-Gui Fang

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  1. Xin Sun
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Correspondence to Jing-Gui Fang.

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Sun, X., Korir, N.K., Han, J. et al. Characterization of grapevine microR164 and its target genes. Mol Biol Rep 39, 9463–9472 (2012). https://doi.org/10.1007/s11033-012-1811-9

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