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UDP-glucuronosyltransferase 1A4 (UGT1A4) polymorphisms in a Jordanian population

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Abstract

Glucuronidation is one of the most important phase II metabolic pathways. It is catalyzed by a family of UDP-glucuronosyltransferase enzymes (UGTs). One of the subfamilies is UGT1A. Allele frequencies in UGT1A4 differ among ethnic groups. The aim of this study was to determine the allelic frequency of two most common defective alleles: UGT1A4*2 and UGT1A4*3 in a Jordanian population. A total of 216 healthy Jordanian Volunteers (165 males and 51 females) were included in this study. Genotyping for UGT1A4*1, UGT1A4*2 and UGT1A4*3 was done using a well established polymerase chain reaction–restriction fragment length polymorphism test. Among 216 random individuals studied for UGT1A4*2 mutation there were 26 individuals who were heterozygous, giving a prevalence of 12% and an allele frequency of 6.5%. Only one individual was homozygous for UGT1A4*2. The UGT1A4*3 mutation was detected as heterozygous in 9 of 216 individuals indicating a prevalence of 4.2% and allele frequency of 3.5%. Three individuals were homozygous for the UGT1A4*3 indicating a prevalence of 1.4%. The prevalence of UGT1A4*2 is similar to the Caucasians but different from other populations whilst the UGT1A4*3 prevalence in the Jordanian population is distinct from other populations. Our results provide useful information for the Jordanian population and for future genotyping of Arab populations in general.

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Conflict of interest

The authors report no conflict of interest.

Author information

Authors and Affiliations

  1. Faculty of Pharmacy, University of Jordan, Amman, 11942, Jordan

    Nancy Hakooz, Sameh Alzubiedi & Al-Motassem Yousef

  2. Faculty of Pharmacy, Zarqa University, Zarqa, 13132, Jordan

    Nancy Hakooz

  3. Jordan Center for Pharmaceutical Research, Amman, 11195, Jordan

    Tawfiq Arafat

  4. Biology Department, Hashemite University, P.O. Box 150459, Zarqa, 13115, Jordan

    Rana Dajani

  5. Faculty of Medicine, University of Jordan, Amman, 11942, Jordan

    Nidaa Ababneh & Said Ismail

Authors
  1. Nancy Hakooz
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  2. Sameh Alzubiedi
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  3. Al-Motassem Yousef
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  4. Tawfiq Arafat
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  5. Rana Dajani
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  6. Nidaa Ababneh
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  7. Said Ismail
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Corresponding author

Correspondence to Nancy Hakooz.

Additional information

This work has been carried out during sabbatical leave granted to the author (Nancy Hakooz) from the University of Jordan during the academic year 2010–2011.

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Hakooz, N., Alzubiedi, S., Yousef, AM. et al. UDP-glucuronosyltransferase 1A4 (UGT1A4) polymorphisms in a Jordanian population. Mol Biol Rep 39, 7763–7768 (2012). https://doi.org/10.1007/s11033-012-1615-y

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  • DOI: https://doi.org/10.1007/s11033-012-1615-y

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