Skip to main content
SpringerLink
Log in

Identification and characterization of genes related to the development of breast muscles in Pekin duck

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Pekin Duck is world-famous for its fast growth, but its breast muscle development is later and breast muscle content is lower compared with other muscular ducks. Therefore, it is very important to discover the genetic mechanism between breast muscle development and relative gene expression in Pekin duck. In current study, the genes which have relationships with breast muscle development were identified by suppression subtractive hybridization. A total of 403 positive clones were sequenced and 257 unigenes were obtained. The expression of 23 genes were analyzed in the breast muscle of 2-, 4-, 6-, 8- week old Pekin ducks. The results showed that unknown clone A233, C83 and C99 showed descending tendency as age increased; KBTBD10, HSPA8, MYL1, ZFP622, MARCH4, Nexilin, FABP4 and MUSTN1 had high expression levels at 6 weeks old; WAC, NT5C3, HSP90AA1, MRPL33, KLF6, TSNAX, CDC42EP3, HSPA4, TRAK1, NR2F2, HAUS1 and IGF1 had high expression levels at 8 weeks and showed ascending tendency as age increased. Expression of these 23 genes were also analyzed in breast muscle, leg muscle, heart, kidney, liver, muscular stomach and sebum cutaneum in 4–8-week old Pekin duck and results showed that most of these genes had high expression in breast muscle, leg muscle and heart.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Zheng Q, Zhang Y, Chen Y, Yang N, Wang X, Zhu D (2009) Systematic identification of genes involved in divergent skeletal muscle growth rates of broiler and layer chickens. BMC Genomics 10:87–99

    Article  PubMed  Google Scholar 

  2. Huang Y, Haley CS, Wu F, Hu F, Hao J, Wu C, Li N (2007) Genetic mapping of quantitative trait loci affecting carcass and meat quality traits in Beijing ducks (Anas platyrhynchos). Anim Genet 38:114–119

    Article  PubMed  CAS  Google Scholar 

  3. Kanisicak O, Mendez JJ, Yamamoto S, Yamamoto M, Goldhamer JD (2009) Progenitors of skeletal muscle satellite cells express the muscle determination gene. Dev Biol 332:131–141

    Article  PubMed  CAS  Google Scholar 

  4. Li L, Liu HH, Xu F, Si JM, Jia J, Wang JW (2010) MyoD expression profile and developmental differences of leg and breast muscle in Peking duck (Anas platyrhynchos Domestica) during embryonic to neonatal stages. Micron 41:847–852

    Article  PubMed  CAS  Google Scholar 

  5. Wang JW, Li L, Han CC, Huang KL, Si JM, He H, Xu F (2011) Molecular evolutionary analysis of the duck MYOD gene family and its differential expression pattern in breast muscle development. Br Poult Sci 4:423–431

    Google Scholar 

  6. Olsson AH, Rönn T, Elgzyri T, Hansson O, Eriksson KF, Groop L, Vaag A, Poulsen P, Ling C (2011) The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes. Mol Genet Metab 103:275–281

    Article  PubMed  CAS  Google Scholar 

  7. Maiti D, Xu ZH, Duh E (2008) Vascular endothelial growth factor induces MEF2C and MEF2-dependent activity in endothelial cells. IOVS 49:3640–3648

    Google Scholar 

  8. Buckingham M, Bajard L, Chang T, Daubas P, Hadchouel J, Meilhac S, Montarras D, Rocancourt D, Relaix F (2003) The formation of skeletal muscle: from somite to limb. J Anat 202:59–68

    Article  PubMed  Google Scholar 

  9. Draghia AR, Brandon MP, Anne LM, Ellis KJ, Schwartz RL, Nordstrom J (2002) Enhanced animal growth via ligand–regulated GHRH myogenic–injectable vectors. FASEB 16:426–428

    Google Scholar 

  10. Wu Y, Liu XL, Xiao HW, Wang J, Jiang F, Zhao S, Liu Y (2009) Expression pattern and prokaryotic expression for Peking duck insulin-like growth factor-I in Escherichia coli. Biochem Genet 47:802–811

    Article  PubMed  CAS  Google Scholar 

  11. Chandra V, Kumar GS, Sharma GT (2011) Temporal expression pattern of insulin-like growth factors (IGF-1 and IGF-2) ligands and their receptors (IGF-1R and IGF-2R) in buffalo (Bubalus bubalis) embryos produced in vitro. Livest Sci 135:225–230

    Article  Google Scholar 

  12. Lee SJ, McPherron AC (1999) Myostatin and the control of skeletal muscle mass. Curr Opin Genet Dev 9:604–607

    Article  PubMed  CAS  Google Scholar 

  13. Gu Z, Zhang Y, Shi P, Zhang YP, Zhu D, Li H (2004) Comparison of avian myostatin genes. Anim Genet 35:470–472

    Article  PubMed  CAS  Google Scholar 

  14. Zhao N, Hou SS, Liu XL, Yang XG, Huang W (2010) Six single nucleotide polymorphisms in adipocyte fatty acid-binding protein (A-FABP) gene in Beijing ducks. Czech J Anim Sci 35:398–400

    Google Scholar 

  15. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 − ΔΔC(T). Methods 25:402–408

    Article  PubMed  CAS  Google Scholar 

  16. Joana PG, Sara CM, Arlindo LO (2009) BiGGEsTS: integrated environment for biclustering analysis of time series gene expression data. BMC Research Notes 124:1–11

    Google Scholar 

  17. Fabien LG, Michael AR (2007) Skeletal muscle satellite cells and adult myogenesis. Curr Opin Cell Biol 19:628–633

    Article  Google Scholar 

  18. Richard B (1990) Interaction between satellite cells and skeletal muscle fibers. Development 109:943–952

    Google Scholar 

  19. Kimberly AH, Scott B, Hui Z, Roland RR (2010) Early response of heat shock proteins to functional overload of the soleus and plantaris in rats and mice. Exp Physiol 95:1145–1155

    Article  Google Scholar 

  20. Michael DC, Jeffry DS, Cynthia FC, William CE, Richard AR (1997) Insulin is degraded extracellularly in wounds by insulin-degrading enzyme. Am J Physiol Endocrinol Metab 273:657–664

    Google Scholar 

  21. Hossein FR, Andrej N, Ashraf K, Maria F, Juleen RZ (2000) Insulin-degrading enzyme identified as a candidate diabetes susceptibility gene in GK rats. Hum Mol Genet 14:2149–2158

    Google Scholar 

  22. Carricaburu VK, Lamia EL, Favereaux L, Payrastre B, Cantley LC, Rame LE (2003) The phosphatidylinositol (PI)-5-phosphate 4-kinase type II enzyme controls insulin signaling by regulating PI-3,4,5-trisphosphate degradation. Proc Natl Acad Sci USA 100:9867–9872

    Article  PubMed  CAS  Google Scholar 

  23. Katja AL, Odile DP, Young BK, Lucia ER, Barbara BK, Lewis CC (2004) Increased insulin sensitivity and reduced adiposity in phosphatidylinositol 5-phosphate 4-kinase ß-/- Mice. Mol Cell Biol 11:5080–5087

    Google Scholar 

  24. Harney DF, Butler RK, Edwards RJ (2005) Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach. Theor Biol Med Model 2:12–17

    Article  PubMed  CAS  Google Scholar 

  25. Baumann CA, Ribon V, Kanzaki M, Thurmond DC, Mora S, Shigematsu S, Bickel PE, Pessin JE, Saltiel AR (2002) CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature 407:202–207

    Google Scholar 

  26. Lin WH, Chiu KC, Chang HM, Lee KC, Tai TY, Chuang LM (2001) Molecular scanning of the human sorbin and SH3-domain-containing-1 (SORBS1) gene: positive association of the T228A polymorphism with obesity and type 2 diabetes. Hum Mol Genet 10:1753–1760

    Article  PubMed  CAS  Google Scholar 

  27. Michael AP, James GR, Gordon SL, George EM (2009) Expression profiling of skeletal muscle following acute and chronic β2-adrenergic stimulation: implications for hypertrophy, metabolism and circadian rhythm. BMC Genomics 10:448–468

    Article  Google Scholar 

  28. Chao LC, Zhang Z, Pei L, Saito T, Tontonoz P, Pilch PF (2007) Nur77 coordinately regulates expression of genes linked to glucose metabolism in skeletal muscle. Mol Endocrinol 21:2152–2163

    Article  PubMed  CAS  Google Scholar 

  29. Pearen MA, Ryall JG, Maxwell MA, Ohkura N, Lynch GS, Muscat GE (2006) The orphan nuclear receptor, NOR-1, is a target of betaadrenergic signaling in skeletal muscle. Endocrinology 147:5217–5227

    Article  PubMed  CAS  Google Scholar 

  30. Pearen MA, Myers SA, Raichur S, Ryall JG, Lynch GS, Muscat GE (2008) The orphan nuclear receptor, NOR-1, a target of {beta}-adrenergic signaling, regulates gene expression that controls oxidative metabolism in skeletal muscle. Endocrinology 149:2853–2865

    Article  PubMed  CAS  Google Scholar 

  31. Maxwell MA, Cleasby ME, Harding A, Stark A, Cooney GJ, Muscat GE (2005) Nur77 regulates lipolysis in skeletal muscle cells. Evidence for cross-talk between the beta-adrenergic and an orphan nuclear hormone receptor pathway. J Biol Chem 280:12573–12584

    Article  PubMed  CAS  Google Scholar 

  32. Carlos I, Makoto PM, Esther A, Michael R, Manuel M (2008) Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR. BMC Mol Biol 9:28–39

    Article  Google Scholar 

  33. Azalina Z, Kien HC, Norhazira AR, Suzana M (2010) Effect of experimental treatment on GAPDH mRNA expression as a housekeeping gene in human diploid fibroblasts. BMC Mol Biol 11:59–65

    Article  Google Scholar 

  34. Adams GR, Haddad F (1996) The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy. J Appl Physiol 81:2509–2516

    PubMed  CAS  Google Scholar 

  35. Chakravarthy MV, Davis BS, Booth FW (2000) IGF-I restores satellite cell proliferative potential in immobilized old skeletal muscle. J Appl Physiol 89:1365–1379

    PubMed  CAS  Google Scholar 

  36. Angela C, Raquel Q, Marcel A, Ramona NP (2010) Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits. BMC Genomics 11:372–386

    Google Scholar 

  37. Shogo H, Tomoko H, Atsuko T, Hideki O, Shinji S, Masaaki T, Kenji O, Fumio M, Hideyuki M (2008) Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle. BMC Genet 9:84–90

    Google Scholar 

  38. Liu C, Gersch RP, Hawke TJ, Hadjiargyrou M (2010) Silencing of Mustn1 inhibits myogenic fusion and differentiation. Am J Physiol Cell Physiol 298:1100–1108

    Article  Google Scholar 

  39. Kostek MC, Chen YW, Cuthbertson DJ, Shi R, Fedele MJ, Esser KA, Rennie MJ (2007) Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1 and FBXO32. Physiol Genomics 31:42–52

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The work was financially supported by China Agriculture Research System (CARS-43-1, CARS-43-42).

Author information

Authors and Affiliations

  1. State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, CAAS, No. 2, YuanMingYuan West Road, HaiDian, 100193, Beijing, People’s Republic of China

    Tieshan Xu, Wei Huang & Shuisheng Hou

  2. Tropical Crops Genetic Resources Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, 571737, Hainan, People’s Republic of China

    Tieshan Xu & Hanlin Zhou

  3. Institute of Animal Science & Veterinary, Hainan Academy of Agriculture Science, Haikou, 571100, Haikou, People’s Republic of China

    Baoguo Ye

  4. College of Animal Science, Henan University of Science and Technology, Luoyang, 471003, He’nan, People’s Republic of China

    Xiaohui Zhang

Authors
  1. Tieshan Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaohui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Baoguo Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hanlin Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shuisheng Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shuisheng Hou.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLS 29 kb)

Supplementary material 2 (XLS 33 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, T., Huang, W., Zhang, X. et al. Identification and characterization of genes related to the development of breast muscles in Pekin duck. Mol Biol Rep 39, 7647–7655 (2012). https://doi.org/10.1007/s11033-012-1599-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-012-1599-7

Keywords

Search

Navigation