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Cloning and molecular characterization of a gene encoding late embryogenesis abundant protein from Pennisetum glaucum: protection against abiotic stresses

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Abstract

Late embryogenesis abundant (LEA) protein family is a large protein family that protects other proteins from aggregation due to desiccation or osmotic stresses. A cDNA clone encoding a group 7 late embryogenesis abundant protein, termed PgLEA, was isolated from Pennisetum glaucum by screening a heat stress cDNA library. PgLEA cDNA encodes a 176 amino acid polypeptide with a predicted molecular mass of 19.21 kDa and an estimated isoelectric point of 7.77. PgLEA shares 70–74% sequence identity with other plant homologs. Phylogenetic analysis revealed that PgLEA is evolutionarily close to the LEA 7 group. Recombinant PgLEA protein expressed in Escherichia coli possessed in vitro chaperone activity and protected PgLEA-producing bacteria from damage caused by heat and salinity. Positive correlation existed between differentially up-regulated PgLEA transcript levels and the duration and intensity of different environmental stresses. In silico analysis of the promoter sequence of PgLEA revealed the presence of a distinct set of cis-elements and transcription factor binding sites. Transcript induction data, the presence of several putative stress-responsive transcription factor binding sites in the promoter region of PgLEA, the in vitro chaperone activity of this protein and its protective effect against heat and salt damage in E. coli suggest a role in conferring abiotic stress tolerance in plants.

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Abbreviations

EST:

Expressed sequence tag

IPTG:

Isopropyl β-d-1-thiogalactopyranoside

LEA:

Late embryogenesis abundant

Pg:

Pennisetum glaucum

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Acknowledgments

This study was supported in part by the Department of Biotechnology (Ministry of Science and Technology, Government of India) in terms of a research Grant and from internal Grants of ICGEB.

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Authors and Affiliations

  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110 067, India

    Palakolanu Sudhakar Reddy, Guda Maheedhar Reddy, Prachi Pandey, Kottakota Chandrasekhar & Malireddy K. Reddy

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  1. Palakolanu Sudhakar Reddy
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  2. Guda Maheedhar Reddy
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  3. Prachi Pandey
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Correspondence to Malireddy K. Reddy.

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Reddy, P.S., Reddy, G.M., Pandey, P. et al. Cloning and molecular characterization of a gene encoding late embryogenesis abundant protein from Pennisetum glaucum: protection against abiotic stresses. Mol Biol Rep 39, 7163–7174 (2012). https://doi.org/10.1007/s11033-012-1548-5

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