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Evaluation of ERα and VDR gene polymorphisms in relation to bone mineral density in Turkish postmenopausal women

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Abstract

It has been suggested that the estrogen receptor alpha (ERα) and vitamin D receptor (VDR) genes as possibly implicated in reduced bone mineral density (BMD) in osteoporosis. The present study investigated the relation of ERα PvuII/XbaI polymorphisms and VDR FokI/TaqI polymorphisms with BMD in Turkish postmenopausal women. Eighty-one osteoporotic and 122 osteopenic postmenopausal women were recruited. For detection of the polymorphisms, polymerase chain reaction-restriction fragment lenght polymorphism techniques have been used. BMD was measured at the lumbar spine and hip by dual-energy X-ray absorptiometry. Distributions of ERα (PvuII dbSNP: rs2234693, XbaI dbSNP: rs9340799) and VDR genotypes (FokI dbSNP rs10735810, TaqI dbSNP: rs731236) were similar in study population. Although overall prevalence of osteoporosis had no association with these genotypes, the prevalence of decreased femoral neck BMD values were higher in the subjects with ERα PvuII “PP” and ERα XbaI “XX” genotypes than in those with “Pp/pp” genotypes and “xx” genotype, respectively (P < 0.05). Furthermore, subjects with VDR FokI “FF” genotype had lower BMD values of femoral neck and total hip compared to those with “Ff” genotype (P < 0.05). In the logistic regression analysis, we confirmed the presence of relationships between the VDR FokI “FF” genotypes, BMI ≤ 27.5, age ≥ 55 and the increased risk of femoral neck BMD below 0.8 value in postmenopausal women. The present data suggests that the ERα PvuII/XbaI and VDR FokI polymorphisms may contribute to the determination of bone mineral density in Turkish postmenopausal women.

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Abbreviations

ERα :

Estrogen receptor alpha

VDR:

Vitamin D receptor

BMD:

Bone mineral density

PCR-RFLP:

Polymerase chain reaction-restriction fragment lenght polymorphism

SNP:

Single nucleotid polymorphism

1,25(OH)2D3 :

1α,25-dihydroxyvitamin D3

BMI:

Body mass index

HWE:

Hardy–Weinberg equilibrium

DXA:

Dual energy X-ray absorptiometry

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Acknowledgment

The present work was supported by the Scientific Research Projects Coordination Unit of Istanbul University (Project No: T-68/15122006).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmacy, Istanbul University, Beyazit, P.O. Box 34116, Istanbul, Turkey

    Ozlem Kurt & Ayse Can

  2. Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey

    Hulya Yilmaz-Aydogan & Mehmet Fatih Seyhan

  3. Department of Phsycial Medicine and Rehabilitation, Uskudar State Hospital, Istanbul, Turkey

    Mehmet Uyar

  4. Department of Medical Biology, Medical Faculty, Yeditepe University, Istanbul, Turkey

    Turgay Isbir

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  1. Ozlem Kurt
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  2. Hulya Yilmaz-Aydogan
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  5. Mehmet Fatih Seyhan
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  6. Ayse Can
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Correspondence to Ayse Can.

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Kurt, O., Yilmaz-Aydogan, H., Uyar, M. et al. Evaluation of ERα and VDR gene polymorphisms in relation to bone mineral density in Turkish postmenopausal women. Mol Biol Rep 39, 6723–6730 (2012). https://doi.org/10.1007/s11033-012-1496-0

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  • DOI: https://doi.org/10.1007/s11033-012-1496-0

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