Skip to main content
SpringerLink
Log in

Distribution and linkage disequilibrium analysis of polymorphisms of MC4R, LEP, H-FABP genes in the different populations of pigs, associated with economic traits in DIV2 line

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

PCR-RFLP was used to analyze the polymorphisms of MC4R, LEP, H-FABP genes in a swine breed composite (DIV2) and 4 swine breeds (Yorkshire, Landrace, Meishan, Bamei). The association study of these polymorphisms with several economic traits was carried out on a DIV2 population. The results obtained showed that MC4R/TaqI genotype had an effect for average backfat thickness (P < 0.05) and lean meat percentage (P < 0.05). At locus LEP/HinfI animals of AA genotype had lower test daily gain than that of BB (P < 0.01) or AB genotype (P < 0.05). At the H-FABP/HaeIII locus lean meat percentage of the individuals with genotype DD were higher than that with genotype dd (P < 0.05). Linkage disequilibrium analysis among MC4R, LEP and H-FABP revealed that these genes were independent. This represented two or more genes that could be combined together within one genotype in order to facilitate breeding for objective traits. In addition, a method allowing simultaneous detection of fragments of MC4R and LEP gene was developed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Dekkers JCM, Hospital F (2002) The use of molecular genetics in the improvement of agricultural populations. Nat Rev Genet 3(1):22–32

    Article  PubMed  CAS  Google Scholar 

  2. Francia E, Tacconi G, Crosatti C, Barabaschi D, Bulgarelli D, Dall’Aglio E, Vale G (2005) Marker assisted selection in crop plants. Plant Cell Tissue Organ Cult 82(3):317–342. doi:10.1007/s11240-005-2387-z

    Article  CAS  Google Scholar 

  3. Houston RD, Cameron ND, Rance KA (2004) A melanocortin-4 receptor (MC4R) polymorphism is associated with performance traits in divergently selected large white pig populations. Anim Genet 35(5):386–390. doi:10.1111/j.1365-2052.2004.01182.x

    Article  PubMed  CAS  Google Scholar 

  4. Kim KS, Larsen N, Short T, Plastow G, Rothschild MF (2000) A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mamm Genome 11(2):131–135. doi:MG99-706[pii]

    Article  PubMed  CAS  Google Scholar 

  5. Campfield LA, Smith FJ, Guisez Y, Devos R, Burn P (1995) Recombinant mouse ob protein: evidence for a peripheral signal linking adiposity and central neural networks. Science 269(5223):546–549

    Article  PubMed  CAS  Google Scholar 

  6. Friedman JM, Halaas JL (1998) Leptin and the regulation of body weight in mammals. Nature 395(6704):763–770. doi:10.1038/27376

    Article  PubMed  CAS  Google Scholar 

  7. Neuenschwander S, Rettenberger G, Meijerink E, Jorg H, Stranzinger G (1996) Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids. Anim Genet 27(4):275–278

    Article  PubMed  CAS  Google Scholar 

  8. de Oliveira Peixoto J, Facioni Guimaraes SE, Savio Lopes P, Menck Soares MA, Vieira Pires A, Gualberto Barbosa MV, de Almeida Torres R, de Almeida ESM (2006) Associations of leptin gene polymorphisms with production traits in pigs. J Anim Breed Genet 123(6):378–383. doi:10.1111/j.1439-0388.2006.00611.x

    Article  PubMed  Google Scholar 

  9. Veerkamp J, Maatman R (1995) Cytoplasmic fatty acid-binding proteins: their structure and genes. Prog Lipid Res 34(1):17

    Article  PubMed  CAS  Google Scholar 

  10. Gerbens F, Rettenberger G, Lenstra JA, Veerkamp JH, te Pas MF (1997) Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene. Mamm Genome 8(5):328–332

    Article  PubMed  CAS  Google Scholar 

  11. Gerbens F, van Erp AJ, Harders FL, Verburg FJ, Meuwissen TH, Veerkamp JH, te Pas MF (1999) Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. J Anim Sci 77(4):846–852

    PubMed  CAS  Google Scholar 

  12. Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  13. Liu BH (1998) Statistical genomics: linkage, mapping and QTL analysis. CRC Press, LLC, Boca Raton, pp 404–409

    Google Scholar 

  14. Burgos C, Carrodeguas JA, Moreno C, Altarriba J, Tarrafeta L, Barcelona JA, Lopez-Buesa P (2006) Allelic incidence in several pig breeds of a missense variant of pig melanocortin-4 receptor (MC4R) gene associated with carcass and productive traits; its relation to IGF2 genotype. Meat Sci 73(1):144–150. doi:10.1016/j.meatsci.2005.11.007

    Article  PubMed  CAS  Google Scholar 

  15. Weir B (1996) Genetic data analysis II. Sinauer, Sunderland

    Google Scholar 

  16. Stachowiak M, Szydlowski M, Obarzanek-Fojt M, Switonski M (2006) An effect of a missense mutation in the porcine melanocortin-4 receptor (MC4R) gene on production traits in polish pig breeds is doubtful. Anim Genet 37(1):55–57. doi:10.1111/j.1365-2052.2005.01373.x

    Article  PubMed  CAS  Google Scholar 

  17. Chen M, Wang AG, Fu JL, Li N (2004) Different allele frequencies of MC4R gene variants in Chinese pig breeds. Archiv Fur Tierzucht-Arch Anim Breed 47(5):463–468

    CAS  Google Scholar 

  18. Karlskov-Mortensen P, Bruun CS, Braunschweig MH, Sawera M, Markljung E, Enfalt AC, Hedebro-Velander I, Josell A, Lindahl G, Lundstrom K, von Seth G, Jorgensen CB, Andersson L, Fredholm M (2006) Genome-wide identification of quantitative trait loci in a cross between Hampshire and Landrace I: carcass traits. Anim Genet 37(2):156–162. doi:10.1111/j.1365-2052.2005.01405.x

    Article  PubMed  CAS  Google Scholar 

  19. Jokubka R, Maak S, Kerziene S, Swalve HH (2006) Association of a melanocortin 4 receptor (MC4R) polymorphism with performance traits in Lithuanian white pigs. J Anim Breed Genet 123(1):17–22

    Article  PubMed  CAS  Google Scholar 

  20. Park HB, Carlborg O, Marklund S, Andersson L (2002) Melanocortin-4 receptor (MC4R) genotypes have no major effect on fatness in a large white × wild boar intercross. Anim Genet 33(2):155–157

    Article  PubMed  CAS  Google Scholar 

  21. Kim KS, Reecy JM, Hsu WH, Anderson LL, Rothschild MF (2004) Functional and phylogenetic analyses of a melanocortin-4 receptor mutation in domestic pigs. Domest Anim Endocrinol 26(1):75–86. doi:S0739724003001334[pii]

    Article  PubMed  CAS  Google Scholar 

  22. Kennes YM, Murphy BD, Pothier F, Palin MF (2001) Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Anim Genet 32(4):215–218. doi:age768[pii]

    Article  PubMed  CAS  Google Scholar 

  23. Dragos-Wendrich M, Stratil A, Hojny J, Moser G, Bartenschlager H, Reiner G, Geldermann H (2003) Linkage and QTL mapping for Sus scrofa chromosome 18. J Anim Breed Genet 120:138–143

    Article  CAS  Google Scholar 

  24. Szydlowski M, Stachowiak M, Mackowski M, Kamyczek M, Eckert R, Rozycki M, Switonski M (2004) No major effect of the leptin gene polymorphism on porcine production traits. J Anim Breed Genet 121(3):149–155

    Article  CAS  Google Scholar 

  25. Chang-Long L, Yu-Chun P, He M, Zi-Lin W, Xue-Gen H (2006) Distributions of polymorphism of add1, mc4r, h-fabp gene, associated with imf and bf in three populations in pig. Hereditas:02

  26. Pang WJ, Yang GS, Long HS, Xiang Z, Zhang BJ (2004) Study on PCR-RFLP molecular marker of pig heart fatty acid-binding protein gene. Lett Biotech 15:124–127

    Google Scholar 

  27. Gerbens F, de Koning DJ, Harders FL, Meuwissen TH, Janss LL, Groenen MA, Veerkamp JH, Van Arendonk JA, te Pas MF (2000) The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. J Anim Sci 78(3):552–559

    PubMed  CAS  Google Scholar 

  28. Uleberg E, Wideroe IS, Grindflek E, Szyda J, Lien S, Meuwissen THE (2005) Fine mapping of a QTL for intramuscular fat on porcine chromosome 6 using combined linkage and linkage disequilibrium mapping. J Anim Breed Genet 122(1):1–6

    Article  PubMed  CAS  Google Scholar 

  29. Ovilo C, Oliver A, Noguera JL, Clop A, Barragan C, Varona L, Rodriguez C, Toro M, Sanchez A, Perez-Enciso M, Silio L (2002) Test for positional candidate genes for body composition on pig chromosome 6. Genet Sel Evol 34(4):465–479

    Article  PubMed  Google Scholar 

  30. Pang WJ, Bai L, Yang GS (2006) Relationship among H-FABP gene polymorphism, intramuscular fat content, and adipocyte lipid droplet content in main pig breeds with different genotypes in western china. Yi Chuan Xue Bao 33(6):515–524. doi:S0379-4172(06)60080-2[pii]10.1016/S0379-4172(06)60080-2

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported financially by the National “863” project of P. R. China (2007AA10Z166), National High Technology Development Project (2006BAD01A08-01), and Hainan Province Key Project of Science and Technology (090136).

Author information

Authors and Affiliations

  1. Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou, Hainan, 571100, People’s Republic of China

    Zhe Chao, Feng Wang, Li-Min Wei, Rui-Ping Sun, Hai-Long Liu, Quan-Wei Liu & Xin-Li Zheng

  2. Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Chang-Yan Deng

Authors
  1. Zhe Chao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Feng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chang-Yan Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Li-Min Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rui-Ping Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hai-Long Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Quan-Wei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xin-Li Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xin-Li Zheng.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chao, Z., Wang, F., Deng, CY. et al. Distribution and linkage disequilibrium analysis of polymorphisms of MC4R, LEP, H-FABP genes in the different populations of pigs, associated with economic traits in DIV2 line. Mol Biol Rep 39, 6329–6335 (2012). https://doi.org/10.1007/s11033-012-1454-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-012-1454-x

Keywords

Search

Navigation