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Q36R polymorphism of KiSS-1 gene in Brazilian head and neck cancer patients

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Abstract

The KiSS-1 metastasis-suppressor gene (KiSS-1) product (metastin, kisspeptin) is reported to act after binding with the natural ligand of a G-protein coupled receptor and this gene product inhibits chemotaxis, invasion, and metastasis of cells. The aim of this study was to evaluate the Q36R polymorphism of KiSS-1 in patients with head and neck cancer and to compare the results with healthy individuals and its association with clinicopathological parameters. Gender, age, smoking and alcohol consumption were analyzed for 744 individual (252 head and neck cancer patients and in 522 control individuals). The molecular analysis of these individuals was made after extraction of genomic DNA using the SSCP-PCR technique. This study did not reveal any significant differences in genotype frequencies between healthy individuals and patients with head and neck cancer or with the clinical parameters. This study showed an increase frequency of the Q36R polymorphism in pharyngeal cancer.

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Acknowledgments

The authors wish to thank all those participating in this study, the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Centro Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for their support; Prof. Adília M. Pires Sciarra for her help in writing the text, Prof. Dr. José A. Cordeiro and Prof. Dr. Moacir F. Godoy for their help in statistical analysis, Ana Lívia S. Galbiatti for technical assistance and FAMERP/FUNFARME.

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Authors and Affiliations

  1. Genetics and Molecular Biology Research Unit (UPGEM), Faculdade de Medicina de São José do Rio Preto—FAMERP, São José do Rio Preto, SP, CEP 15090-000, Brazil

    Mariângela Torreglosa Ruiz, Ana Lívia Silva Galbiatti, Érika Cristina Pavarino & Eny Maria Goloni-Bertollo

  2. Universidade Federal do Triângulo Mineiro—UFTM, Av. Getúlio Guaritá, 159, Abadia, Uberaba, MG, 38025-440, Brazil

    Mariângela Torreglosa Ruiz

  3. Otorhinolaryngology and Head and Neck Surgery Department, Faculdade de Medicina de São José do Rio Preto—FAMERP, Av. Brigadeiro Faria Lima, n. 5416, São José do Rio Preto, Brazil

    José Victor Maniglia

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  1. Mariângela Torreglosa Ruiz
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  2. Ana Lívia Silva Galbiatti
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Correspondence to Eny Maria Goloni-Bertollo.

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Ruiz, M.T., Galbiatti, A.L.S., Pavarino, É.C. et al. Q36R polymorphism of KiSS-1 gene in Brazilian head and neck cancer patients. Mol Biol Rep 39, 6029–6034 (2012). https://doi.org/10.1007/s11033-011-1416-8

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  • DOI: https://doi.org/10.1007/s11033-011-1416-8

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