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Porcine MuRF2 and MuRF3: molecular cloning, expression and association analysis with muscle production traits

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Abstract

Muscle specific RING finger protein2 (MuRF2) and Muscle specific RING finger protein3(MuRF3) are two important members of the muscle specific RING finger protein family, which are especially expressed in cardiac and skeletal muscle tissues and play critical roles during the myocyte differentiation, development and morphogenesis. In this study, the molecular characteristics of porcine MuRF2 and MuRF3 gene were reported, and furthermore two variants of MuRF2 were identified. The tissue distribution pattern analyses revealed that MuRF2-b and MuRF3 mRNA was exclusively expressed in striated muscle tissues while MuRF2-a had a low-level expression in liver tissue. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) results displayed MuRF2 mRNA expression levels were significantly varied at three stages of fetal skeletal muscle in Landrace pigs, and the expression of MuRF2-a was lower than that of MuRF2-b in all stages. An essencial region of −396 to −22 for transcription was identified at the 5’UTR of porcine MuRF2 gene, while no active regulatory fragment found in the 5’UTR of mouse MuRF2. A single nucleotide polymorphism (SNP), c.915G > A was identified in MuRF2 exon 5. A HinfI PCR-RFLP was developed for SNP genotyping in two different pig populations. Association of the genotypes with growth and carcass traits showed that different genotypes of MuRF2 were significantly (P < 0.05) associated with average daily gain on test, carcass weight and carcass length. The study suggested that the porcine MuRF2 and MuRF3 genes are involved in the muscle growth and development, and can be considered as potential candidate genes affecting muscle production traits in the pig.

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Abbreviations

CDS:

Coding sequence

ESTs:

Expressed sequence tags

MuRF1:

Muscle specific RING finger protein-1

MuRF2:

Muscle specific RING finger protein-2

MuRF3:

Muscle specific RING finger protein-3

ORF:

Open reading frame

RFLP:

Restriction fragment length polymorphism

SNP:

Single nucleotide polymorphism

UTR:

Un-translated region

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (30771169), Wuhan Youth Chenguang Project (200750731282), the National High Science and Technology Foundation of China “863”, and the creative team project of Education Ministry of China (IRT0831). The authors thank Dr. Max Rothschild for kindly providing DNA samples of Berkshire × Yorkshire pig family.

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Authors and Affiliations

  1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    H. Shen, S. H. Zhao, J. H. Cao, X. Y. Li & B. Fan

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  1. H. Shen
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  2. S. H. Zhao
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  3. J. H. Cao
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  5. B. Fan
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Correspondence to B. Fan.

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Shen, H., Zhao, S.H., Cao, J.H. et al. Porcine MuRF2 and MuRF3: molecular cloning, expression and association analysis with muscle production traits. Mol Biol Rep 38, 5115–5123 (2011). https://doi.org/10.1007/s11033-010-0659-0

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  • DOI: https://doi.org/10.1007/s11033-010-0659-0

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