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Molecular cloning and expression analysis of a novel SANT/MYB gene from Gossypium barbadense

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Abstract

MYB family transcription factors are implicated in multiple developmental processes. Herein, a new full-length cDNA encoding a SANT/MYB transcription factor (designated as GbRL2) was cloned and characterized from cotton (Gossypium barbadense L.) for the first time. The full-length cDNA of GbRL2 was 573 bp with a 240 bp open reading frame (ORF) encoding a deduced protein of 80 amino acid polypeptide with a calculated molecular mass of 8.96 kDa and an isoelectric point of 8.96. Sequence alignment revealed that GbRL2 had high homology with other single SANT/MYB domain containing genes, including the RADIALIS genes in Antirrhinum majus and Bournea leiophylla. Semi-quantitative reverse transcript polymerase chain reaction (RT-PCR) revealed that at seedling stage, GbRL2 was strongly expressed in leaves but merely in stems. In opening flowers, the expression of GbRL2 was moderate in the petals but could not be detected in stamens. In ovules, the expression of GbRL2 could not be detected at −3 days post-anthesis (DPA) but increased during early elongation stage (0 DPA, +3 DPA, +5 DPA and +8 DPA). The transcripts of GbRL2 could also be detected at +8 DPA elongating fibers. We also examined the expression of RL2 gene in Gossypium hirstum cultivar Xu-142 and its fuzzless-lintless-seed mutant fl plants. The GhRL2 gene was ectopically expressed at −3 DPA in the fl mutant while the expression of GhRL2 in WT could not be detected. The expression of GhRL2 decreased early (+5 DPA) while that of WT was still strong. Our results suggest that GbRL2 may participate in development of various organs and may be a target for genetic improvement of cotton fiber.

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Abbreviations

ORF:

Open-reading frame

RT-PCR:

Reverse transcript polymerase chain reaction

DPA:

Days post-anthesis

RACE:

Rapid amplification of cDNA ends

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Acknowledgments

This work was funded by the China National '973' Program on Key Basic Research Project (2007CB108805), transgenic plant project (2009 ZX08009-091B), 863 project (2008 AA 10Z 180) and Shanghai Leading Academic Discipline Project (B2009).

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Authors and Affiliations

  1. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Fei Zhang, Xiang Liu, Kaijing Zuo & Kexuan Tang

  2. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, People’s Republic of China

    Xiaofen Sun

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  1. Fei Zhang
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  2. Xiang Liu
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  3. Kaijing Zuo
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  4. Xiaofen Sun
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  5. Kexuan Tang
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Correspondence to Kexuan Tang.

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Zhang, F., Liu, X., Zuo, K. et al. Molecular cloning and expression analysis of a novel SANT/MYB gene from Gossypium barbadense . Mol Biol Rep 38, 2329–2336 (2011). https://doi.org/10.1007/s11033-010-0366-x

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  • DOI: https://doi.org/10.1007/s11033-010-0366-x

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