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Identification and expression profile of GbAGL2, a C-class gene from G ossypium barbadense

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Abstract

An AGAMOUS (AG)-like gene, GbAGL2, was isolated from Gossypium barbadense and characterized. Alignment and phylogenetic analysis indicated that GbAGL2 shared high homology with AG-subfamily genes and belonged to a C-class gene family. DNA gel blot analysis showed that GbAGL2 belonged to a low-copy gene family. Reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) revealed that GbAGL2 was highly expressed in reproductive tissues including ovules and carpels, but barely expressed in vegetative tissues. In addition, GbAGL2 expression in a cotton cultivar XuZhou142 (wt) (XZ142, G. hirsutum L.) and its fibreless mutant XZ142 (fl) was examined. RNA in situ hybridization analysis indicated that GbAGL2 transcripts were preferentially restricted to outer ovule integuments, carpels and fibres. These expression patterns implied that GbAGL2 might participate in the development of the carpel and ovule. Furthermore, Arabidopsis transformation was performed and modifications occurred in flowers, and the silique length of transgenic plants also increased slightly, suggesting that the GbAGL2 gene may have a positive effect on the development of the ovary or ovule. Our findings suggest that GbAGL2 might not only specify the identity of floral organs but also play a potential key role in ovary or fibre development in cotton.

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Abbreviations

AG:

AGAMOUS

bp:

base pair

dpa:

days post anthesis

fl:

fuzzless-lintless

RACE:

rapid amplification of cDNA end

qPCR:

quantitative real-time polymerase chain reaction

RT-PCR:

reverse transcription PCR

SEM:

scanning electron microscopy

Wt:

wild-type

XZ142:

XuZhou142

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Authors and Affiliations

  1. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Xiang Liu, Kaijing Zuo, Fei Zhang, Ying Li, Jieting Xu, Lida Zhang & Kexuan Tang

  2. State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China

    Xiaofen Sun & Kexuan Tang

Authors
  1. Xiang Liu
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  2. Kaijing Zuo
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  3. Fei Zhang
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  4. Ying Li
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  5. Jieting Xu
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  6. Lida Zhang
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  7. Xiaofen Sun
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  8. Kexuan Tang
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Correspondence to Kexuan Tang.

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Supplementary tables and figures pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/Dec2009/pp941-951/suppl.pdf

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Liu, X., Zuo, K., Zhang, F. et al. Identification and expression profile of GbAGL2, a C-class gene from G ossypium barbadense . J Biosci 34, 941–951 (2009). https://doi.org/10.1007/s12038-009-0108-1

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  • DOI: https://doi.org/10.1007/s12038-009-0108-1

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