Abstract
Elongation factors (EF) are abundant cell proteins that play important roles in the metabolism of all multicellular organisms. Here we describe a functional analysis of elongation factor 1-alpha (EF1A) and elongation factor 2 (EF2), from the Pacific white shrimp, Litopenaeus vannamei. Full-length cDNAs of genes corresponding to EF1A and EF2 were obtained that were 1547 and 2729 bp long, with open reading frames encoding 461 and 846 amino acids, respectively. The deduced amino acid sequences of L. vannamei EF1A and EF2 showed high similarity with those from mice, humans, chickens and other shrimps. RT-PCR analysis indicated that mRNA transcripts of EF1A and EF2 are strongly (but differentially) expressed in haemocytes and gill tissue, and at varying levels in other examined tissues, of the shrimps. Levels of both EF1A and EF2 transcripts increased when shrimps were challenged by pH and cadmium stress, but reached maximal levels after different exposure periods. These results indicate that EF1A and EF2 may play distinct, essential roles in the repair of cellular damage induced by pH and cadmium stress.
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Acknowledgments
This research was supported by the National Natural Science Foundation of China (Grant nos. 30570287 and 30970455), and the Natural Science Foundation of the Guangdong Province in the P.R. China (Grant nos. 06025052 and 8151063101000035), and Guangdong Provincial Oceanic Fisheries Science and Technology Project (Grant no. A200899D01) and the Project of the Department of Education of Guangdong Province for the Young Researcher of Higher School. We are grateful for the help of Profs. Qili Feng and Sichun Zheng from the laboratory of Molecular Entomology, South China Normal University.
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Lei Wang, Yuan Liu contributed equally to this work.
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Wang, L., Liu, Y., Wang, WN. et al. Molecular characterization and expression analysis of elongation factors 1A and 2 from the Pacific white shrimp, Litopenaeus vannamei . Mol Biol Rep 38, 2167–2178 (2011). https://doi.org/10.1007/s11033-010-0345-2
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DOI: https://doi.org/10.1007/s11033-010-0345-2