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MYBBP1A: a new Ipr1’s binding protein in mice

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Abstract

Infection with mycobacterium tuberculosis (MTB) can cause different outcomes in hosts with variant genetic backgrounds. Previously, we identified an intracellular pathogen resistance 1 (Ipr1) gene with the role of resistance of MTB infection in mice model. However, until now, its binding proteins have been little known even for its human homology, SP110. In this study, the homology for mouse Ipr1 in canines was found to have an extra domain structure, h.1.5.1. And 30 potential candidate proteins were predicted to bind canine Ipr1, which were characterized of the interacting structure with the h.1.5.1. Among them, MYBBP1A was verified to bind with both Ipr1 and eGFP-Ipr1 in mouse macrophage J774A.1 clone 21 cells using co-immunoprecipitation method. And with the constructed high-confidence Ipr1-involved network, we suggested that Ipr1 might be involved in apoptosis pathway via MYBBP1A.

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Acknowledgement

We gratefully acknowledge Dr. Gustavo Mostoslavsky for his advices on the construction of lentivirus vectors and Dr. Jianmin Wu for his advices on the construction of PPI networks. This work was supported by grants AI49421 and P01 AI056296 from the National Institutes of Health and by the National Basic Research Program of China (Grant No. 2009CB918700).

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Authors and Affiliations

  1. Department of Immunology and Infectious Diseases, Harvard School of Public Health, 667 Huntington Avenue, Boston, MA, 02115, USA

    Lei Cai, Hui Pan, Krzysztof Trzciński, Claudette M. Thompson & Igor Kramnik

  2. Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai JiaoTong University, 800 Dongchuan Road, 200240, Shanghai, China

    Lei Cai & Qiang Wu

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  1. Lei Cai
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  2. Hui Pan
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Correspondence to Lei Cai.

Electronic supplementary material

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Supplementary file 1

Primary canine Ipr1’s candidate binding proteins (XLS 1249 kb)

Supplementary file 2

30 canine Ipr1’s potential binding candidate proteins (XLS 218 kb)

Supplementary file 3

The network function analysis results (XLS 34 kb)

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Cai, L., Pan, H., Trzciński, K. et al. MYBBP1A: a new Ipr1’s binding protein in mice. Mol Biol Rep 37, 3863–3868 (2010). https://doi.org/10.1007/s11033-010-0042-1

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  • DOI: https://doi.org/10.1007/s11033-010-0042-1

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