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Establishment of an effective virus induced gene silencing system with BSMV in Haynaldia villosa

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Abstract

In recent years, virus-induced gene-silencing (VIGS) has shown to be a powerful reverse genetics tool for gene function study. In this paper, an effective and persistent virus-induced gene silencing (VIGS) system was established with barley stripe mosaic virus (BSMV) for Haynaldia villosa. Examination of GFP gene expression showed that the BSMV vector moved systemically from leaf to leaf in inoculated H. villosa plants. This vector, expressing a cDNA fragment of phytoene desaturase (PDS), suppressed the transcript level of endogenous Hv-PDS gene as early as 6 days after inoculation, and caused photobleaching symptoms mainly on the newly developed upper leaves. Moreover, PDS gene silencing phenotype persisted through the whole growing period in H. villosa. With this established VIGS system, function analysis of a powdery mildew resistance related gene Hv-LRR was successfully performed. This is the first report that BSMV can be used for VIGS in a wild relative species of wheat. The established VIGS system will be a powerful reverse genetics tool for gene function study in H. villosa, an important genetic resource for wheat breeding.

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Acknowledgements

This research was supported by the National Basic Research Program of China (2009CB118304), the High Tech Program of China (2006AA10A104), the National Natural Science Foundation of China (30700503), the Jiangsu Province Natural Science Foundation (BK2007163) and the Ministry of Educate 111 Project (B08025).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, 210095, Nanjing, China

    Xiaoyun Wang, Aizhong Cao, Xiue Wang & Peidu Chen

  2. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Chunmei Yu & Daowen Wang

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  1. Xiaoyun Wang
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  2. Aizhong Cao
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  3. Chunmei Yu
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  4. Daowen Wang
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  5. Xiue Wang
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  6. Peidu Chen
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Correspondence to Peidu Chen.

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Xiaoyun Wang and Aizhong Cao have contributed equally to this paper.

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Wang, X., Cao, A., Yu, C. et al. Establishment of an effective virus induced gene silencing system with BSMV in Haynaldia villosa . Mol Biol Rep 37, 967–972 (2010). https://doi.org/10.1007/s11033-009-9766-1

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  • DOI: https://doi.org/10.1007/s11033-009-9766-1

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