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The sensitization of glioma cells to cisplatin and tamoxifen by the use of catechin

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Abstract

Telomerase expression strongly correlates with the grade of malignancy in glioma with inhibition illustrating a definite increase in chemosensitivity. This study was designed to investigate the effects of a green tea derivative, epigallocatechin-3-gallate (EGCG); together with either cisplatin or tamoxifen in glioma, and to investigate whether these effects are mediated through telomerase suppression. EGCG showed a significant cytotoxic effect on 1321N1 cells after 24 h and on U87-MG cells after 72 h (P < 0.001) without significantly affecting the normal astrocytes. Treatment with EGCG inhibited telomerase expression significantly (P < 0.01) and enhanced the effect of cisplatin and tamoxifen in both 1321N1 (P < 0.01) and U87-MG (P < 0.001) cells. EGCG, as a natural product has enormous potential to be an anti-cancer agent capable of enhancing tumour cell sensitivity to therapy.

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Acknowledgement

This work was supported by a grant from the University of Central Lancashire.

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Authors and Affiliations

  1. Brain Tumour North West, Faculty of Science, University of Central Lancashire, Preston, UK

    Amal Shervington, Vidya Pawar, Sharad Menon, Dipti Thakkar & Rahima Patel

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  1. Amal Shervington
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  2. Vidya Pawar
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  3. Sharad Menon
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  4. Dipti Thakkar
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  5. Rahima Patel
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Correspondence to Amal Shervington.

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Shervington, A., Pawar, V., Menon, S. et al. The sensitization of glioma cells to cisplatin and tamoxifen by the use of catechin. Mol Biol Rep 36, 1181–1186 (2009). https://doi.org/10.1007/s11033-008-9295-3

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  • DOI: https://doi.org/10.1007/s11033-008-9295-3

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