Abstract
Galectin-9, a member of galectin family, plays multiple roles in a variety of cellular functions, including cell adhesion, aggregation, and apoptosis. Galectin-9 also has three isoforms (named galectin-9L, galectin-9M, and galectin-9S), but whether these isoforms differ in their functions remains poorly understood. In this study, we showed that transient expression of galectin-9L decreased E-selectin levels, while transient expression of galectin-9M or galectin-9S increased E-selectin levels in LoVo cells, which do not express endogenous galectin-9. We also found that over-expression of three galectin-9 isoforms led to increased attachment of LoVo cells to extracellular matrix proteins respectively, while over-expression of galectin-9M or galectin-9S increased the adhesion of LoVo cells to human umbilical vein endothelial cells in vitro. In summary, these findings indicate that different isoforms of galectin-9 exhibit distinct biological functions.
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Acknowledgements
We thank Dr. Lai Wang from University of Texas, Southwestern Medical Center, Dallas for his great help in preparing the manuscript. This work was funded by the Terry Fox Foundation for Cancer Research.
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Zhang, F., Zheng, M., Qu, Y. et al. Different roles of galectin-9 isoforms in modulating E-selectin expression and adhesion function in LoVo colon carcinoma cells . Mol Biol Rep 36, 823–830 (2009). https://doi.org/10.1007/s11033-008-9251-2
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DOI: https://doi.org/10.1007/s11033-008-9251-2