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Introduction of G1 phase arrest in Human Hepatocellular carcinoma cells (HHCC) by APMCF1 gene transfection through the down-regulation of TIMP3 and up-regulation of the CDK inhibitors p21

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Abstract

We previously found that there was up-regulation of APMCF1 expression in apoptotic MCF-7 cells. Moreover, bioinformatics analysis has found that APMCF1 molecules had similar size and structure with molecules which belong to small G-protein superfamily. We presume that APMCF1 plays certain biological role in the regulation of cell proliferation and apoptosis. In this study, we first detected the expression pattern of APMCF1 in human hepatocellular carcinoma cell line and find no expression in Human Hepatocellular carcinoma cells (HHCC) and enhanced expression in HepG2 cells. Expression of liposome-mediated ectogenic APMCF1 induced inhibition of HHCC growth and cell cycle, and RNAi inhibited APMCF1 expression and promoted HepG2 cell growth. Results of cell cycle gene chips analysis showed up-regulation of p21 expression and down-regulation of TIMP3 in HHCC cells expressing ectogenic APMCF1, indicating that APMCF1 participates at least partially in cell cycle regulation through regulating genes such as p21 and TIMP3.

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Abbreviations

APMCF1:

Apoptosis related of MCF-7 cells

HHCC:

Human Hepatocellular carcinoma cells

TIMP3:

Tissue inhibitor of metalloproteinases 3

DMEM:

Dulbecco’s-modified Eagle medium

MTT:

Methylthiazolyldiphenyl-tetrazolium bromide

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Acknowledgements

We thank Chendan, Wuyousheng for assistance with our experiments. This work was supported by the National Natural Science Foundation of China, No.30270667.

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Authors and Affiliations

  1. Department of Pathology, State Key Laboratory of GI Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, Shaanxi Province, China

    Qinlong Li, Wei Yan, Shuangping Guo, Wenyong Wang, Zhipei Zhang, Li Wang, Jing Zhang & Wenliang Wang

  2. Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, Shaanxi Province, China

    Shengquan Cheng

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  1. Qinlong Li
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  2. Wei Yan
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  7. Li Wang
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Correspondence to Wei Yan.

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Li, Q., Yan, W., Cheng, S. et al. Introduction of G1 phase arrest in Human Hepatocellular carcinoma cells (HHCC) by APMCF1 gene transfection through the down-regulation of TIMP3 and up-regulation of the CDK inhibitors p21. Mol Biol Rep 33, 257–263 (2006). https://doi.org/10.1007/s11033-006-9007-9

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  • DOI: https://doi.org/10.1007/s11033-006-9007-9

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