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Breeding for carrot resistance to Alternaria dauci without compromising taste

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Abstract

Developing carrot varieties highly resistant to Alternaria dauci is a top priority for breeders. Meanwhile, consumers are increasingly demanding as regards final product quality, particularly taste. Bitterness is one of the five common taste sensations, but it is rejected by most carrot consumers. Therefore, providing tools for efficient plant breeding of resistant, low bitter carrots would be helpful. While resistance QTLs (rQTLs) have already been identified for carrot resistance to A. dauci, the genetic control and mapping of the metabolites involved in bitterness perception have not been addressed so far. We identified the metabolites most involved in bitterness by combining chemical and sensory analyses of a set of resistant and susceptible carrot genotypes grown in different environments. We evaluated their genetic control and heritability in a segregating F2:3 population over 2 years of field trials and searched for colocalizations between rQTLs and metabolite QTLs (mQTLs) to evaluate the link between bitterness and resistance traits. Our results suggest that it is possible to increase resistance while favoring low bitter varieties by selecting genomic regions involved in the expression of one or the other trait and counter-selecting others when r- and mQTL colocalization is unfavorable.

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Acknowledgements

The authors would like to thank the IRHS-Imorphen team involved in tunnel experiments in Angers, Emilie Clavel and François Villeneuve for the field trial in Ctifl (Lanxade) and Stéphan Plas (Invénio) for the field experiment in Les Landes. This work is part of the research program AlterQual financed by the Ministry of Agriculture (FranceAgriMer).

Funding

This study was funded by the Ministry of Agriculture (FranceAgriMer).

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Authors and Affiliations

  1. IRHS, Agrocampus Ouest, INRA, Université d’Angers, SFR 4207 QuaSaV, 49071,, Beaucouzé, France

    Valérie Le Clerc, Claire Yovanopoulos, Mathilde Piquet, Anita Suel, Sébastien Huet, Claude Koutouan, Latifa Hamama, Besnik Pumo & Mathilde Briard

  2. Ctifl, centre de Saint-Rémy-de-Provence, Route de Mollèges, 13210, Saint Rémy de Provence, France

    Christophe Aubert, Guillaume Chalot & Michel Jost

  3. Ctifl, centre de Balandran, 751, Chemin de Balandran, 30127, Bellegarde, France

    Valentine Cottet

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  1. Valérie Le Clerc
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  2. Christophe Aubert
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  3. Valentine Cottet
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  4. Claire Yovanopoulos
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Correspondence to Valérie Le Clerc.

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Le Clerc, V., Aubert, C., Cottet, V. et al. Breeding for carrot resistance to Alternaria dauci without compromising taste. Mol Breeding 39, 59 (2019). https://doi.org/10.1007/s11032-019-0966-7

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