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Molecular tools for detecting Pdh1 can improve soybean breeding efficiency by reducing yield losses due to pod shatter

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Abstract

Pod shattering is an ancestral trait that promotes seed dispersal; however, shattering can have substantial yield losses in cultivated soybean. During the improvement process, American soybean breeders virtually eliminated the shatter phenotype for released varieties, but in other countries, such as Ghana, shatter persists. The objective of our research was to find a molecular tool to implicate genetic shatter susceptibility, validate its usefulness, and apply this knowledge to identify shattering potential in parental lines. Previous research revealed the gene Pdh1 on chromosome 16 plays a crucial role in determining the shatter phenotype. A perfect molecular marker assay was developed to detect alleles of the Pdh1 gene. A genome-wide association study (GWAS) was performed using the Pdh1 allele status as a phenotype and identified a highly associated marker in the SoySNP50K array. Soybean accessions from the National Plant Germplasm System (GRIN-NPGS) with shatter score and SoySNP50K data were evaluated to determine the impact of the predicted Pdh1 alleles on early and late pod shattering. An online tool was developed to enable researchers to query the GRIN collection for the predicted Pdh1 allele status. Lines from an African soybean germplasm collection were analyzed, and it was determined that 22.5% of lines had the shatter-susceptible alleles of Pdh1; two of seven Ghanaian released soybean varieties had the shatter-susceptible alleles of Pdh1. Soybean breeding programs that access germplasm from the GRIN or the African collection can utilize these resources to eliminate the Pdh1 effects on pod shatter and thus improve yield potential.

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Funding

This work was supported by the US Department of Agriculture-Agricultural Research Service. This research is in part funded by the USAID Feed the Future Lab for Soybean Value Chain Research. The award number is Cooperative Agreement Number AID OAA-L-14-00001.

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Authors and Affiliations

  1. Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA

    Carrie Miranda

  2. Division of Biochemistry, University of Missouri, Columbia, MO, 65211, USA

    Carolyn Culp

  3. Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic

    Mária Škrabišová

  4. Department of Health Management and Informatics and MU Informatics Institute, University of Missouri, Columbia, MO, 65211, USA

    Trupti Joshi

  5. Département de Phytologie and Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, QC, G1V 0A6, Canada

    François Belzile

  6. USDA/ARS Corn Insects and Crop Genetics Research, Ames, IA, 50011, USA

    David M. Grant

  7. USDA/ARS Plant Genetics Research Unit, Columbia, MO, 65211, USA

    Kristin Bilyeu

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  1. Carrie Miranda
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  2. Carolyn Culp
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  3. Mária Škrabišová
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Correspondence to Kristin Bilyeu.

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Miranda, C., Culp, C., Škrabišová, M. et al. Molecular tools for detecting Pdh1 can improve soybean breeding efficiency by reducing yield losses due to pod shatter. Mol Breeding 39, 27 (2019). https://doi.org/10.1007/s11032-019-0935-1

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