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Identification and molecular genetic mapping of Chili veinal mottle virus (ChiVMV) resistance genes in pepper (Capsicum annuum)

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Abstract

Chili veinal mottle virus (ChiVMV) threatens the agricultural production of peppers (Capsicum annuum) in Asia and Africa. In this study, we evaluated ChiVMV resistance in the four pepper varieties CV3, CV4, CV8, and CV9. Segregation analyses revealed that CV3 and CV8 contain the single dominant resistance gene Cvr1, and CV9 contains the single recessive resistance gene cvr4. SNP markers were developed and used to map the Cvr1 gene in CV3 to the short arm of chromosome 6 where NLR genes are clustered. In CV4 oligogenic resistance loci were detected. A genotyping-by-sequencing (GBS) combined with modified sliding window approach mapped two resistance loci, to chromosomes 6 and 10. The development of SNP markers and the resulting knowledge of genomic positioning will assist in breeding ChiVMV-resistant pepper varieties and in the fine mapping of ChiVMV resistance genes.

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Acknowledgements

This work was supported by a grant (710001-07) from the Vegetable Breeding Research Center through Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs and Golden Seed Project (213006-05-1-SB540), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Service (KFS), Republic of Korea.

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  1. Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-921, South Korea

    Joung-Ho Lee, Jeong-Tak An, Muhammad Irfan Siddique, Koeun Han, Seula Choi, Jin-Kyung Kwon & Byoung-Cheorl Kang

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  1. Joung-Ho Lee
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  2. Jeong-Tak An
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  3. Muhammad Irfan Siddique
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  4. Koeun Han
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  5. Seula Choi
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  7. Byoung-Cheorl Kang
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Correspondence to Byoung-Cheorl Kang.

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Lee, JH., An, JT., Siddique, M.I. et al. Identification and molecular genetic mapping of Chili veinal mottle virus (ChiVMV) resistance genes in pepper (Capsicum annuum). Mol Breeding 37, 121 (2017). https://doi.org/10.1007/s11032-017-0717-6

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