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Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepper

  • Published:
Molecules and Cells

Abstract

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinai mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum ‘Dempsey’ containing an eIF4E mutation (pvr1 2) and C. annuum ‘Perennial’ containing an eIFiso4E mutation (pvr6). C. annuum ‘Dempsey’ was susceptible and C. annuum ‘Perennial’ was resistant to ChiVMV. All F1 plants showed resistance, and F2 individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five F2 and 329 F3 plants of 17 families were genotyped with pvr1 2 and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both pvr1 2 and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in eIF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of F2 plants revealed that all plants containing homozygous genotypes of both pvr1 2 and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of eIF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

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Authors and Affiliations

  1. Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    JeeNa Hwang, Jinjie Li, Wing-Yee Liu, Song-Ji An & Byoung-Cheorl Kang

  2. Molecular Markers Diagnostics Laboratory, Plant Breeding and NongWoo Bio Ltd., Suwon, 443-807, Korea

    Hwajin Cho

  3. Plant Pathology Laboratory, NongWoo Bio Ltd., Suwon, 443-807, Korea

    Nam Han Her

  4. Plant Breeding and Genetics, Cornell University, Cornell, USA

    Inhwa Yeam

  5. National Institute of Horticultural and Herbal Science, Suwon, 440-706, Korea

    Dosun Kim

Authors
  1. JeeNa Hwang
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  2. Jinjie Li
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  3. Wing-Yee Liu
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  4. Song-Ji An
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  5. Hwajin Cho
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  6. Nam Han Her
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  7. Inhwa Yeam
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  8. Dosun Kim
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  9. Byoung-Cheorl Kang
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Correspondence to Byoung-Cheorl Kang.

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These authors contributed equally to this work.

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Hwang, J., Li, J., Liu, WY. et al. Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepper. Mol Cells 27, 329–336 (2009). https://doi.org/10.1007/s10059-009-0042-y

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  • DOI: https://doi.org/10.1007/s10059-009-0042-y

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