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OsDSR-1, a calmodulin-like gene, improves drought tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.)

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Abstract

Calmodulin-like (CML) genes regulate plant growth, development, and responses to abiotic stresses such as salinity and drought. Many genes encoding CML proteins have been identified from rice (Oryza sativa), but their functions remain largely unknown. Our characterization of one putative CML gene, OsDSR-1 (O. sativa Drought Stress Response-1), showed that its protein binds Ca2+ and displays Ca2+-dependent conformational changes. In contrast to wild-type (WT) and OsDSR-1-RNA interference (OsDSR-1-Ri) plants, transgenic rice plants that overexpress OsDSR-1 were significantly more drought tolerant and had increased sensitivity to abscisic acid. Furthermore, their concentrations of free proline and soluble sugars and the activities of reactive oxygen species-scavenging enzymes as well as the transcript levels of many ROS-scavenging and stress-related genes were significantly enhanced under drought stress. Much less hydrogen peroxide and malondialdehyde accumulated in OsDSR-1-overexpressing (OsDSR-1-OE) plants than in either the Ri or WT plants. All of these results suggest that OsDSR-1 plays important roles in conferring tolerance to drought in rice by decreasing the occurrence of oxidative damage.

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Abbreviations

ABA:

Abscisic acid

CAT:

Catalase

Ca2+ :

Calcium ion

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

OE:

Overexpressing

OsDSR-1 :

Oryza sativa Drought Stress Response-1

OsDSR-1-OE:

OsDSR-1-overexpressing

OsDSR-1-Ri:

OsDSR-1-RNA interference

POD:

Peroxidase

Ri:

RNA interference

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This research was supported by the Science and Technology Key Project of Hunan Province, People’s Republic of China (S2011F2022).

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Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, People’s Republic of China

    Xuming Yin, Lifang Huang, Manling Wang, Yanchun Cui & Xinjie Xia

  2. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, People’s Republic of China

    Xinjie Xia

Authors
  1. Xuming Yin
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  2. Lifang Huang
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  3. Manling Wang
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  4. Yanchun Cui
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  5. Xinjie Xia
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Contributions

YXM constructed the vector, generated the transgenic plants, performed the abiotic stress treatments and validation experiments, and wrote the manuscript; HLF determined the concentrations of free proline soluble sugars, H2O2, and MDA and evaluated the Ca2+-binding property of the recombinant protein; WML measured the activities of CAT, POD, and SOD, and assessed ABA sensitivity; CYC analyzed the expression of stress-related genes and analyzed the data; and XXJ designed the experimental plan and revised the manuscript. All of the authors agreed on the content of the paper and declare no conflicting interests.

Corresponding author

Correspondence to Xinjie Xia.

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Yin, X., Huang, L., Wang, M. et al. OsDSR-1, a calmodulin-like gene, improves drought tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.). Mol Breeding 37, 75 (2017). https://doi.org/10.1007/s11032-017-0668-y

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  • DOI: https://doi.org/10.1007/s11032-017-0668-y

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