Abstract
Sustainability of the cranberry industry is threatened by widespread and increasing losses due to fruit rot in the field as well as increasing restrictions on fungicide inputs. Breeding for resistance offers a partial solution but is challenging because fruit rot is caused by a complex of pathogenic fungi that can vary by location and from year to year. We identified four genetically diverse germplasm accessions that exhibit broad-spectrum fruit rot resistance under field conditions. Three of these accessions were used in biparental crosses to develop four populations segregating for resistance. Genotyping by sequencing was used to generate single-nucleotide polymorphism (SNP) markers for development of high-density genetic maps and quantitative trait locus (QTL) analyses. Nineteen QTL associated with fruit rot resistance, distributed on nine linkage groups, were discovered in our populations. Three of these QTL matched previously reported fruit rot resistance QTL. Four newly reported QTL found on linkage group 8 (Vm8), which explain between 21 and 33% of the phenotypic variance for fruit rot, are of particular interest to our breeding program. The populations described herein were also phenotyped for other horticulturally important traits, and QTL associated with yield and berry weight were identified. These QTL provide markers for candidate gene discovery and for future breeding efforts to enhance and pyramid disease resistance and other traits into elite horticultural backgrounds.
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Acknowledgments
We are grateful to Josh Honig and Jennifer Vaiciunas for help with developing and running the SSRs and Kristia Adams, Karen DeStefano, and Sue Vancho for technical and field support. This project was supported by the USDA-SCRI under Grant 2008-51180-04878; the USDA National Institute of Food and Agriculture under the Agriculture and Food Research Initiative Competitive Grant USDA-NIFA-2013-67013-21107; Ocean Spray Cranberries, Inc.; NJ Cranberry & Blueberry Research Council.
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Daverdin, G., Johnson-Cicalese, J., Zalapa, J. et al. Identification and mapping of fruit rot resistance QTL in American cranberry using GBS. Mol Breeding 37, 38 (2017). https://doi.org/10.1007/s11032-017-0639-3
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DOI: https://doi.org/10.1007/s11032-017-0639-3