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Meloidogyne incognita nematode resistance QTL in carrot

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Abstract

Root-knot nematodes (Meloidogyne spp.) are major pests attacking carrots (Daucus carota) worldwide, causing galling and forking of the storage roots, rendering them unacceptable for market. Genetic resistance could significantly reduce the need for broad-spectrum soil fumigants in carrot production. In this study, genetic resistance to Meloidogyne incognita was mapped. Three diverse sources of resistance, from Syria (HM), Europe (SFF) and South America (Br1091), were identified. Two F2 mapping populations were developed using these parents, (Br1091 × HM1) and (SFF × HM2), as well as a segregating population derived from the self-pollination of a HM plant (HM3). Analysis revealed four QTLs conditioning resistance in Br1091 × HM1, three in SFF × HM2, and three in HM3. A consensus genetic map of the three populations revealed five non-overlapping QTLs for M. incognita resistance, one each on carrot chromosomes 1, 2, 4, 8, and 9. One QTL was present in all three populations, in the same region of chromosome 8 as Mj-1 which imparts resistance to M. javanica.

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Acknowledgments

This work was funded by the California Fresh Carrot Advisory Board grants (to PWS and PAR); Specialty Crop Research Initiative award 2008-51180-04896 (to PWS and PAR); Organic Agriculture Research and Extension Initiative award 2011-51300-30903 (to PWS and PAR); and a Monsanto Graduate Research Fellowship to J.P. through the University of Wisconsin-Madison Plant Breeding and Plant Genetics Program. The authors appreciate the capable assistance provided by Dr. Doug Senalik.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin – Madison, 1575 Linden Drive, Madison, WI, 53706, USA

    Joshua Parsons, Massimo Iorizzo & Philipp Simon

  2. Department of Nematology, University of California, Riverside, CA, 92521, USA

    William Matthews & Philip Roberts

  3. Vegetable Crops Research Unit, USDA-ARS, 1575 Linden Drive, Madison, WI, 53706, USA

    Philipp Simon

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  1. Joshua Parsons
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  2. William Matthews
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  3. Massimo Iorizzo
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Correspondence to Philipp Simon.

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Parsons, J., Matthews, W., Iorizzo, M. et al. Meloidogyne incognita nematode resistance QTL in carrot. Mol Breeding 35, 114 (2015). https://doi.org/10.1007/s11032-015-0309-2

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