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Development of tightly linked markers and identification of candidate genes for Fusarium crown rot resistance in barley by exploiting a near-isogenic line-derived population

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This study demonstrates the feasibility of developing co-segregating markers and identifying candidate genes for Fusarium crown rot resistance in barley based on the generation and exploitation of a near-isogenic line-derived large population.

Abstract

Fusarium crown rot (FCR) is a chronic and severe disease in cereals in semi-arid regions worldwide. Previous studies showed that FCR assessment could be affected by many factors including plant height, growth rate as well as drought stress. Thus, accurate assessment, which is essential for detailed mapping of any locus conferring FCR resistance, is difficult. Targeting one of the resistance loci reported earlier, we developed a near-isogenic line-derived population consisting of 1820 F9 lines. By analysing this population, the Qcrs.cpi-4H locus was mapped to an interval of 0.09 cM covering a physical distance of about 637 kb and 13 markers co-segregating with the targeted locus were developed. Candidate genes underlying the resistance locus were identified by analysing the expression and sequence variation of genes in the targeted interval. The accurate localization and the development of co-segregating markers should facilitate the incorporation of this large-effect QTL into breeding programmes as well as the cloning of gene(s) underlying the locus.

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Acknowledgements

The work reported here was supported by the Commonwealth Scientific and Industrial Organization (CSIRO), Australia (Project code: R-10191-01). YJ is grateful to the Sichuan Agricultural University and the China Scholarship Council for funding his visit to CSIRO. AH is grateful to University of Tasmania, Australia, and Khulna University, Bangladesh, for financial supports during the tenure of his PhD studentship.

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Authors and Affiliations

  1. Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, St. Lucia, QLD, 4067, Australia

    Yunfeng Jiang, Ahsan Habib, Zhi Zheng & Chunji Liu

  2. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

    Yunfeng Jiang, Yuming Wei & You-Liang Zheng

  3. School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, Private Bag 54, Hobart, TAS, 7001, Australia

    Ahsan Habib & Meixue Zhou

  4. Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, 9208, Bangladesh

    Ahsan Habib

  5. National Foxtail Millet Improvement Centre, Institute of Millet Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, China

    Zhi Zheng

Authors
  1. Yunfeng Jiang
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  2. Ahsan Habib
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  3. Zhi Zheng
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  4. Meixue Zhou
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  5. Yuming Wei
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  6. You-Liang Zheng
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  7. Chunji Liu
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Correspondence to Chunji Liu.

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Communicated by Kevin Smith.

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Jiang, Y., Habib, A., Zheng, Z. et al. Development of tightly linked markers and identification of candidate genes for Fusarium crown rot resistance in barley by exploiting a near-isogenic line-derived population. Theor Appl Genet 132, 217–225 (2019). https://doi.org/10.1007/s00122-018-3209-0

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  • DOI: https://doi.org/10.1007/s00122-018-3209-0

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