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Quantitative trait loci increasing acylsugars in tomato breeding lines and their impacts on silverleaf whiteflies

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Abstract

Solanum pennellii LA716, a wild relative of tomato, produces acylsugars, an insect resistance compound with activity against many tomato insect pests. Breeding of cultivated tomato using S. pennellii LA716 as a donor parent has led to the development of the elite acylsugar-producing tomato breeding line CU071026. CU071026 contains five introgressed S. pennellii genomic regions, and produces acylsugars at moderate levels that are effective against insect pests. A BC1F1 population was created by crossing the F1 CU071026 × S. pennellii LA716 with CU071026 as the recurrent parent; this BC1F1 population was used to identify additional regions of the S. pennellii genome important for further improvement of acylsugar production. This population was genotyped with 94 markers in the segregating regions and phenotyped for level of acylsugar production. Using QTLNetwork 2.1 for the detection of quantitative trait loci (QTL) and epistatic interactions, this study identified five QTL for total acylsugar level. Additionally, two epistatic interactions between QTL were found to control significant levels of total acylsugar production. Two of the QTL identified were further evaluated in silverleaf whitefly (Bemisia tabaci) field cage trials using acylsugar breeding lines that differ for the presence/absence of these QTL. While high levels of silverleaf whitefly resistance were observed in all acylsugar breeding lines, lines containing the additional QTL on either chromosomes 6 or 10 had increased levels of total acylsugar production and reduced incidence of whitefly. Acylsugar lines containing the chromosome 6 QTL also had increased density of the type IV glandular trichomes which produce and exude acylsugars.

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Acknowledgments

We would like to thank for George Kennedy for advice prior to the insect cage experiment and critical reading of the manuscript. We thank Larry Robertson for loan of the cages used for the insect study. We would also like to thank Jean-Luc Jannink for the valuable discussions on epistasis. This study was funded by the Hatch Project NYC-149440) and the Cornell University Vegetable Breeding Institute.

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The authors have declared no conflict of interests.

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  1. Department of Plant Breeding and Genetics, Cornell University, 301 Bradfield Hall, Ithaca, NY, 14853, USA

    Brian M. Leckie, Darlene M. De Jong & Martha A. Mutschler

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  1. Brian M. Leckie
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  2. Darlene M. De Jong
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  3. Martha A. Mutschler
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Correspondence to Martha A. Mutschler.

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Leckie, B.M., De Jong, D.M. & Mutschler, M.A. Quantitative trait loci increasing acylsugars in tomato breeding lines and their impacts on silverleaf whiteflies. Mol Breeding 30, 1621–1634 (2012). https://doi.org/10.1007/s11032-012-9746-3

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