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Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.)

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Abstract

Spotted leaf 5 (spl5), a lesion mimic mutant, was first identified in rice (Oryza sativa L.) japonica cv. Norin8 in 1978. This mutant exhibits spontaneous disease-like lesions in the absence of any pathogens and resistance to rice blast and bacterial blight; however, the target gene has not yet been isolated. In the present study, we employed a map-based cloning strategy to finely map the spl5 gene. In an initial mapping with 100 F2 individuals (spl5/spl5) derived from a cross between the spl5 mutant and indica cv. 93-11, the spl5 gene was located in a 3.3-cM region on chromosome 7 using six simple sequence repeat (SSR) markers. In a high-resolution genetic mapping, two F2 populations with 3,149 individuals (spl5/spl5) were derived from two crosses between spl5 mutant and two indica cvs. 93-11 and Zhefu802 and six sequence-tagged site (STS) markers were newly developed. Finally, the spl5 gene was mapped to a region of 0.048 cM between two markers SSR7 and RM7121. One BAC/PAC contig map covering these markers’ loci and the spl5 gene was constructed through Pairwise BLAST analysis. Our bioinformatics analysis shows that the spl5 gene is located in the 80-kb region between two markers SSR7 and RM7121 with a high average ratio of physical to genetic distance (1.67 Mb/cM) and eighteen candidate genes. The analysis of these candidate genes indicates that the spl5 gene represents a novel class of regulators controlling cell death and resistance response in plants.

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Acknowledgments

The authors thank the reviewers for helpful scientific comments. This work was supported by the National Natural Science Foundation of China (Grants no. 30370867 and 30771329), National Key Programs for Transgenic Crops (2008ZX08009-001, 003; 2009ZX08009-076B) and Zhejiang Normal University Innovative Research Team Program.

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Authors and Affiliations

  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China

    Xifeng Chen, Jianwei Pan, Yang Jin, Zhimin Gu & Bojun Ma

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Xifeng Chen, Jing Cheng, Guanghuai Jiang & Wenxue Zhai

  3. China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China

    Qian Qian

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  1. Xifeng Chen
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  2. Jianwei Pan
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  5. Yang Jin
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  8. Wenxue Zhai
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Correspondence to Wenxue Zhai or Bojun Ma.

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X. Chen, J. Pan and J. Cheng contributed equally to this work.

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Chen, X., Pan, J., Cheng, J. et al. Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.). Mol Breeding 24, 387–395 (2009). https://doi.org/10.1007/s11032-009-9299-2

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