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Construction of intraspecific linkage maps, detection of a chromosome inversion, and mapping of QTL for constitutive root aerenchyma formation in the teosinte Zea nicaraguensis

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Abstract

The teosinte Zea nicaraguensis, a wild relative of maize, possesses a flooding tolerance-related trait: the formation of constitutive root aerenchyma under drained (non-flooded) soil conditions. A previous study suggested that the degree of constitutive aerenchyma formation varies within Z. nicaraguensis. The objectives of this study were to construct linkage maps, to determine the marker order in a region of chromosome 4 in which recombination between maize and Z. nicaraguensis is suppressed, and to identify quantitative trait loci (QTL) controlling constitutive root aerenchyma formation in two segregating populations of Z. nicaraguensis. A total of 236 simple sequence repeat (SSR) markers were screened for polymorphism in an S1 population of Z. nicaraguensis. Seventy-one polymorphic SSR markers were assigned to 10 chromosomes, and a linkage map was constructed covering 793.5 cM. In the S1 map, a paracentric inversion was detected on the long arm of chromosome 4; this rearrangement was confirmed in an S1 linkage map of a different Z. nicaraguensis accession. Composite interval mapping analysis in 96 S1 plants revealed QTL for aerenchyma formation on chromosomes 1 (bins 1.06–1.07) and 7 (bin 7.01), explaining 17 and 12% of the total phenotypic variance, respectively. The QTL on chromosome 1 was verified by using 156 S2 plants. Near-isogenic lines exhibiting the presence or absence of the aerenchyma QTL have been developed that should be useful for genetic and physiological analyses of root aerenchyma formation.

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Acknowledgments

The authors wish to thank the North Central Regional Plant Introduction Station (NCRPIS), USDA-ARS/Iowa State University, Ames, Iowa, USA, for providing seed of Z. nicaraguensis (PI 615697); the International Maize and Wheat Improvement Center (CIMMYT), Mexico, for supplying seed of Z. nicaraguensis (CIMMYT 13451); and the National Agricultural Research Center for Kyushu Okinawa Region for providing maize inbred Mi29. We also thank Dr. B. Kindiger (USDA/ARS) for critically reviewing the manuscript, and Dr. R. McK. Bird (North Carolina State University), Dr. H. Takahashi (Akita Prefectural University), and Dr. C. H. Loaisiga (Universidad Nacional Agraria) for contributing to the work. This work was supported by grants from Sapporo Bioscience Foundation, the Bio-oriented Technology Research Advancement Institution (Promotion of Basic Research Activities for Innovative Biosciences, No. H20/seeds-01-02), and the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, FBW1101).

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Authors and Affiliations

  1. Forage Crop Breeding Research Team, National Institute of Livestock and Grassland Science, Nasushiobara, Tochigi, 329-2793, Japan

    Yoshiro Mano & Fumie Omori

  2. Genetic Resources Unit, Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046, Japan

    Kazuyoshi Takeda

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  1. Yoshiro Mano
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  2. Fumie Omori
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  3. Kazuyoshi Takeda
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Correspondence to Yoshiro Mano.

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Mano, Y., Omori, F. & Takeda, K. Construction of intraspecific linkage maps, detection of a chromosome inversion, and mapping of QTL for constitutive root aerenchyma formation in the teosinte Zea nicaraguensis . Mol Breeding 29, 137–146 (2012). https://doi.org/10.1007/s11032-010-9532-z

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