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Development of microsatellite markers in canary seed (Phalaris canariensis L.)

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Abstract

Annual canarygrass, commonly known as canary seed (Phalaris canariensis L.), is a self-pollinated diploid cereal (2n = 12) with a genome size of 3,800 Mbp. Canary seed is presently used for bird-feed with a potential to develop it for human consumption. Marker-assisted selection can be used to accelerate breeding of new canary seed cultivars. Microsatellites or simple sequence repeat (SSR) markers generally show a high degree of polymorphism in different plant genera. FIASCO (Fast Isolation by AFLP of Sequences COntaining repeats) was used to generate microsatellite markers specific for canary seed. An enriched SSR (AG)17 library derived from DNA isolated from a canary seed cultivar (CDC Togo) was produced. Analysis and DNA sequencing of the library resulted in 744 clones from which 132 primer pairs were designed. Seventy-eight functional markers amplified unique products from canary seed DNA. These SSR markers revealed the biodiversity among a panel of 48 canary seed accessions. Polymorphic information content (PIC) values of 37 polymorphic microsatellites ranged from 0.08 to 0.73 with an average of 0.36.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

EST:

Expressed sequence tag

FIASCO:

Fast isolation by AFLP of sequences containing repeats

PCR:

Polymerase chain reaction

PIC:

Polymorphic information content

RAPD:

Random amplification of polymorphic DNA

SNP:

Single nucleotide polymorphism

SSR:

Simple sequence repeat

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Acknowledgments

This research was conducted with the financial support of the Agriculture, Food and Rural Revitalization (PH & RNC), Canary Seed Growers Association (PH), Canada Research Chair, and Canada Foundation for Innovation (RNC).

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Authors and Affiliations

  1. Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Jingzhao Li, Monica Båga & Ravindra N. Chibbar

  2. Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Pierre Hucl

Authors
  1. Jingzhao Li
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  2. Monica Båga
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  3. Pierre Hucl
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  4. Ravindra N. Chibbar
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Corresponding author

Correspondence to Ravindra N. Chibbar.

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Li, J., Båga, M., Hucl, P. et al. Development of microsatellite markers in canary seed (Phalaris canariensis L.). Mol Breeding 28, 611–621 (2011). https://doi.org/10.1007/s11032-010-9513-2

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