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Functional diversity at the Rc (red coleoptile) gene in bread wheat

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Abstract

The presence of the allele Rc-A1b on chromosome 7A specified the expression profile of the F3h-1 (encoding flavanone 3-hydroxylase) genes and anthocyanin pigmentation in coleoptiles of Russian bread wheat cultivar ‘Saratovskaya 29’. A quantitative RT-PCR analysis compared the temporal expression profile of F3h-A1, F3h-B1, and F3h-D1 in the coleoptiles of ‘Saratovskaya 29’ and the standard cytogenetic stock ‘Chinese Spring’ (‘Hope’ 7A), both of which carry Rc-A1b. There was no within-genotype variation for expression level of the F3h-1 homoeologues at any of the sampling times, but the expression profiles varied markedly between the two genotypes. This result suggested that there may be functional allelic diversity at Rc-A1, which affects the transcription of the F3h-1 genes in colored coleoptiles. Microsatellite-based genetic mapping was used to locate Rc-A1 along with the new loci Pc-A1 (purple culm), Plb-A1 (purple leaf blade), and Pls-A1 (purple leaf sheath) in a single cluster on the short arm of chromosome 7A.

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Acknowledgments

We thank Renate Voss, Annette Marlow, and Marina Baklanova for their excellent technical assistance. The senior author was supported by the Russian Foundation for Basic Research (project No. 08-04-00368-a), the Russian Science Support Foundation, and the Deutsche Forschungsgemeinschaft (project No. BO 1423/9-1/551902).

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  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentjeva ave. 10, 630090, Novosibirsk, Russia

    E. K. Khlestkina & T. A. Pshenichnikova

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany

    M. S. Röder & A. Börner

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  1. E. K. Khlestkina
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  2. M. S. Röder
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  3. T. A. Pshenichnikova
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  4. A. Börner
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Correspondence to E. K. Khlestkina.

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Khlestkina, E.K., Röder, M.S., Pshenichnikova, T.A. et al. Functional diversity at the Rc (red coleoptile) gene in bread wheat. Mol Breeding 25, 125–132 (2010). https://doi.org/10.1007/s11032-009-9312-9

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