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Genetic variation through Dissociation (Ds) insertional mutagenesis system for rice in Korea: progress and current status

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Abstract

A gene detection strategy using two-component Ac/Ds construct, with the mobile Ds transposon, has been developed to better understand gene functions in crops. Currently, 115,000 Ds insertion lines have been generated through the Ac/Ds gene trap system in Korea using japonica rice Dongjin as donor. Four hundred and thirty-seven mutants from 12,162 Ds-tagged lines were catalogued, including physiological and agronomic traits. Different traits were identified with distinct characteristics in terms of tillers, panicles, leaves, flowers, seed, chlorophyll content, and height. Culm and panicle length, number of panicles, and days to flowering of the Dongjin Ds population revealed high standard deviations compared with the donor cultivar. An evaluation of the Ds distribution on the chromosome revealed that 74.5% of the Ds were reinserted into gene-rich regions, making this Ac/Ds-mediated gene trap system useful in helping to gain an understanding of the function of genes and thus improve the gene-tagging system in rice.

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Acknowledgments

This work was supported in part by grants from the twenty-first Century Frontier Program (CG3312) of the Crop Functional Genomics Center and the Biogreen 21 program, Rural Development Administration (RDA), Republic of Korea. We thank the reviewers for the constructive comments and Ms. Tess Rola of the Communication and Publication Services Division, IRRI, for editing the manuscript.

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Authors and Affiliations

  1. Functional Crop Resource Development Division, Department of Functional Crop, NICS, RDA, 1085 Neidong, Milyang, Gyeong-nam, 627-803, Korea

    Dong-Soo Park, Soo-Kwon Park, Sang-Ik Han, Hoe-Jeong Wang, Norvie L. Manigbas, Min-Hee Nam, Hang-Won Kang & Gihwan Yi

  2. Pohang University of Science and Technology, Pohang, Korea

    Young-Min Woo

  3. Dong-A University, Busan, Korea

    Doh-Hoon Kim

  4. Gyeongsang National University, Jinju, Korea

    Nam-Soo Jun & Chang-Deok Han

  5. National Academy of Agricultural Science, RDA, Suwon, 441-707, Korea

    Byoung-Ohg Ahn, Doh-Won Yun, Ung-Han Yoon, Yong-Hwan Kim & Myung-Chul Lee

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  1. Dong-Soo Park
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Correspondence to Gihwan Yi.

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Park, DS., Park, SK., Han, SI. et al. Genetic variation through Dissociation (Ds) insertional mutagenesis system for rice in Korea: progress and current status. Mol Breeding 24, 1–15 (2009). https://doi.org/10.1007/s11032-009-9300-0

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