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A consensus list of microsatellite markers for olive genotyping

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Abstract

Cultivar identification is a primary concern for olive growers, breeders, and scientists. This study was aimed at examining the SSR markers retrieved from the literature and currently used in olive study, in order to select those most effective in characterizing the olive accessions and to make possible the comparison of data obtained by different laboratories. Olive microsatellite profiles were assessed by four independent laboratories, which analyzed 37 pre-selected SSR loci on a set of 21 cultivars. These SSR markers were initially tested for their reproducibility, power of discrimination and number of amplified loci/alleles. Independent segregation was tested for each pair of SSRs in a controlled cross and the allelic error rate was quantified. Some of them were finally selected as the most informative and reliable. Most of the alleles were sequenced and their sizes were determined. Profiles of the reference cultivars and a list of alleles with their sizes obtained by sequencing are reported. Several genetic parameters have been analysed on a larger set of cultivars allowing for a deeper characterization of the selected loci. Results of this study provide a list of recommended markers and protocols for olive genotyping as well as the allelic profile of a set of reference cultivars that would be useful for the establishment of a universal database of olive accessions.

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Acknowledgments

The authors are very grateful to Dr. Juan Caballero, Dr. Carmen del Río and Prof. Diego Barranco, responsible of the evaluation, maintenance and identification of the World Olive Germplasm Bank, Córdoba, Spain, for providing the DNA samples. A special thanks is also due to the other sample providers. The work has been supported by the Project RIOM-MIPAAF “Research and Innovation for Olive growing in Italian Southern regions”, the Project OLIBIO–MIPAAF “Development of competitive, sustainable, traceable and safe systems to produce high quality olive oils by organic agriculture” and the Project FISR “Improving flavor and nutritional properties of plant food after first and second transformation”, Activity ‘Molecular characterization of main olive varieties’.

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Authors and Affiliations

  1. CNR-Istituto di Genetica Vegetale, 06128, Perugia, Italy

    Luciana Baldoni, Nicolò G. Cultrera, Roberto Mariotti, Claudia Ricciolini & Sergio Arcioni

  2. CNR-Istituto di Genetica Vegetale, 50019, Sesto Fiorentino (Firenze), Italy

    Giovanni G. Vendramin & Anna Buonamici

  3. DSGVA, University of Sassari, 07100, Sassari, Italy

    Andrea Porceddu

  4. Dipartimento di Biologia Cellulare e Ambientale, Sez. Biologia Cellulare e Molecolare, University of Perugia, 06100, Perugia, Italy

    Vania Sarri

  5. Departamento de Agronomía, Campus Universitario de Rabanales, University of Córdoba, 14080, Córdoba, Spain

    Maria A. Ojeda, Isabel Trujillo & Luis Rallo

  6. Centro “Alameda del Obispo”-IFAPA, 14083, Córdoba, Spain

    Angjelina Belaj

  7. CRA-Centro di Ricerca per l’Olivicoltura e l’Industria Olearia, 87036, Rende (CS), Italy

    Enzo Perri, Amelia Salimonti & Innocenzo Muzzalupo

  8. CRA-Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, 26900, Lodi, Italy

    Innocenzo Muzzalupo

  9. Istituto di Genomica Applicata, Parco Scientifico e Tecnologico, 33100, Udine, Italy

    Alberto Casagrande & Raffaele Testolin

  10. Dipartimento di Scienze Agrarie e Ambientali, University of Udine, 33100, Udine, Italy

    Orietta Lain, Rachele Messina & Raffaele Testolin

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  1. Luciana Baldoni
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  2. Nicolò G. Cultrera
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  3. Roberto Mariotti
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  13. Angjelina Belaj
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Correspondence to Luciana Baldoni.

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Baldoni, L., Cultrera, N.G., Mariotti, R. et al. A consensus list of microsatellite markers for olive genotyping. Mol Breeding 24, 213–231 (2009). https://doi.org/10.1007/s11032-009-9285-8

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